醋酸钠-醋酸体系DNA、组蛋白复合产生共振光散射衰减测定铝  

The determination of aluminum based on light-scattering decreased in sodium acetate-acetate buffer solution,DNA and histone system

作　　者：刘凤海[1] 杜晓燕[2] 郑晶[2] 吕昌武[1] 肖颖[1] 陈卫东[1] 

机构地区：[1]黑龙江省牡丹江市疾病预防控制中心,黑龙江牡丹江157011 [2]哈尔滨医科大学公共卫生学院,哈尔滨150000

出　　处：《中国卫生检验杂志》2005年第6期646-647,共2页Chinese Journal of Health Laboratory Technology

基　　金：黑龙江省科学技术计划项目(20020101)

摘　　要：目的:探讨微量铝的测定方法。方法:共振光散射衰减法。结果:在pH值5.5,0.25mg/LDNA及40mg/L组蛋白条件下,扫描光谱显示最大光散射波长为332.8nm,铝离子浓度在0.54～4.86mg/L范围内,和光散射强度呈负相关,线性回归方程为Y=10.9-0.0189X,回归系数为0.998,回收率在92%～106%。结论:该方法灵敏、简便、快速,适合进行微量铝离子的测定。Objective:To explore a method for the examination of tittle aluminum.Methods:The light-scattering decreased method.Results:If pH was 5.5,DNA was 0.25 mg/L and histone was 40 mg/L, scan spectrum showed that the maxmal light-scattering wavelength was 332.8 nm.There was a liner relationship between the quantity of aluminum and the intensity of light-scattering.The linear range were 0.54~ 4.86 mg/L and the equation were Y=10.9-0.0189X, the recovery rates were in the range 92%~106%.Conclusion:The method is sensitive,simple and quick, so it is reliable to measure the mount of aluminum.

关 键 词：共振光散射 组蛋白 衰减 DNA 醋酸钠 复合 线性回归方程 测定方法 离子浓度 散射强度 回归系数 微量铝 pH值 负相关 回收率 铝离子 

分 类 号：R446.11[医药卫生—诊断学] R392.11[医药卫生—临床医学]