鼻咽癌组织的显微切割及其RNA线性扩增  

Microdissection and RNA Line-amplification of Nasopharyngeal Carcinoma Tissue

作　　者：周艳宏[1] 曾朝阳[1] 熊炜[1] 罗晓敏[1] 李小玲[1] 范松青[1] 张文玲[1] 刘华英[1] 李征[1] 杨一新[1] 武明花[1] 唐珂[1] 曹利[1] 沈守荣[2] 李桂源[1] 

机构地区：[1]中南大学湘雅医学院肿瘤研究所,长沙410078 [2]中南大学湘雅三医院消化内科,长沙410083

出　　处：《生物化学与生物物理进展》2005年第5期463-467,共5页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目(30300201;30470955;3030560;30200312);新世纪优秀人才支持计划资助项目(NCET-04-0761)~~

摘　　要：从微小体积鼻咽癌活检标本中获取纯净癌细胞一直是鼻咽癌分子生物学研究中的难题.为了寻找一种能从鼻咽癌活检组织中获得高纯度、高质量RNA来完成cDNA微阵列(cDNA Microarray)实验的简便实用方法,采用RNAlater技术保存鼻咽癌活检组织,显微切割技术来获得高纯度鼻咽癌细胞,利用RNA线性扩增技术得到cDNA微阵列实验所需RNA.结果表明:利用RNAlater技术可以很好地保持组织RNA的稳定,通过优化显微切割和RNA线性扩增的条件获得了cDNA微阵列实验所需的高纯度、高质量RNA.It is a difficult problem in molecular biology study of nasopharyngeal carcinoma (NPC) to obtain pureness tumor cells from tiny NPC tissue. To seek a convenient and applied way to obtain pureness and high quality RNA from NPC tissue for cDNA Microarray, the study was designed to use RNA later technology to keep the NPC dissected tissue and obtain pureness NPC cells by microdissection and gain RNA through RNA line amplification technology. The results hinted that RNAlater technology could keep the stability of NPC tissue and obtain the pureness and high quality RNA for cDNA Microarray combined with microdissection and RNA line amplification through optimum conditions.

关 键 词：RNAlater技术 手工显微切割 RNA线性扩增技术 CDNA微阵列 

分 类 号：Q734.2[生物学—分子生物学]