富硒乳酸菌抑制镉诱发小鼠血浆NO和TNF-α释放作用  

Inhibited Effects of Se-enriched Lactobacillus on Plasma NO and TNF-α Release of Mice Induced by Cadmium

作　　者：许龙兵[1] 陈龙[1] 江善祥[2] 周娟[1] 

机构地区：[1]南京师范大学生命科学学院 [2]南京农业大学动物医学院,江苏南京210095

出　　处：《南京师大学报（自然科学版）》2005年第2期75-78,共4页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：江苏省重点实验室开放基金资助项目(KJS02022)

摘　　要：选择48只健康成年雄性小鼠,随机分为对照组(C组)、镉暴露组(Cd组)、镉+低剂量富硒乳酸菌组(Cd-LSe组)、镉+高剂量富硒乳酸菌组(CdHSe组).腹腔注射CdCl2进行镉暴露实验,连续6d.研究富硒乳酸菌对镉暴露小鼠血浆肿瘤坏死因子-α(TNF-α)、一氧化氮(NO)水平及一氧化氮合酶(NOS)活性的影响.结果显示,镉染毒暴露第6d,Cd组血浆NO含量明显高于C组,CdLSe和CdHSe组与C组无明显差异,低于Cd组;Cd组NOS活性高于C、CdLSe和CdHSe组;CdLSe和CdHSe组NOS活性与C组无显著差异性;Cd组iNOS活性明显高于C、Cd-LSe和Cd-HSe组,Cd-LSe和CdHSe组iNOS活性与C组无显著差异;Cd组血浆TNF-α升高,而CdLSe和CdHSe组未见升高,且低于于C组.提示富硒乳酸菌在有效缓解镉诱导血浆iNOS活性上升,NO、TNF-α释放增加介导损伤过程中可能起重要作用.Forty-eight healthy adult male mice are chosen and randomly divided into a control group(group C), a Cd-exposed group(group Cd), a Cd plus low dose Se-enriched lactobacillus group(group Cd-LSe) and a Cd plus high dose Se-enriched lactobacillus group(group Cd-HSe). The Cd-exposed experiment is induced by abdominal injection of CdCl_2 per day over six days. Effects of Se-enriched lactobacillus on plasma Tumor necrosis factor-α(TNF-α), nitric oxide(NO) levels and nitricoxide synthase (NOS) activity in cadmium-exposed mice are studied. The results show that plasma NO content of group Cd is obviously higher than that of group C, and there are lower values in groups Cd-LSe and Cd-HSe than in group Cd on the 6th day post experiment. Plasma NOS activity of group Cd is higher than that of groups C, Cd-LSe and Cd-HSe, and the NOS values of groups Cd-LSe and Cd-HSe have no obvious differences as compared with that of group C. A higher iNOS activity value in group Cd than that in groups C, Cd-LSe and Cd-HSe is investigated, moreover, the iNOS activity of groups Cd-LSe and Cd-HSe have no significant differences as compared with that of group C. Plasma TNF-α level rises in group Cd, however, the values in groups Cd-LSe and Cd-HSe do not increase and is lower than that in group C. It is suggested that Se-enriched lactobacillus may play an important role in the remission of cadmium-induced iNOS activity increases and the massive release of NO and TNF-α.

关 键 词：富硒乳酸菌 镉 一氧化氮 肿瘤坏死因子-Α 

分 类 号：Q592.1[生物学—生物化学]