小檗碱诱导人急性T淋巴细胞白血病细胞凋亡的研究  

The Study of Apoptosis of MOLT-4 Cells Induced by Berberine in Vitro

作　　者：蒋艳[1] 胡群[1] 刘双又[1] 丁玉峰[2] 刘爱国[1] 张柳清[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科,武汉430030 [2]华中科技大学同济医学院附属同济医院药学部,武汉430030

出　　处：《医药导报》2005年第7期568-570,共3页Herald of Medicine

摘　　要：目的探讨小檗碱诱导人急性T淋巴细胞白血病细胞(MOLT-4)凋亡的作用。方法将处于对数生长期的MOLT-4细胞分为3组,A组加入小檗碱(10,100mg·L?1),B组加入柔红霉素1mg·L-1,C组加入小檗碱+柔红霉素,作用6和24h后,用MTT法检测细胞存活率;用流式细胞术、DNA电泳及光学显微镜观察小檗碱对MOLT-4细胞凋亡的影响。结果作用6,24h后,A组(10mg·L-1)细胞存活率分别为81.80%,64.67%;B组为98.38%,38.46%,C组为68.35%,20.51%;C组与A、B组比较,在两个时间段差异均有显著性(P<0.05)。小檗碱诱导MOLT-4细胞发生典型的细胞凋亡形态学变化,出现DNA规律性断裂,电泳呈典型的梯状条带变化。细胞周期分析提示小檗碱浓度在10,100mg·L-1时作用24h细胞凋亡率分别为5.83%,19.93%。结论小檗碱具有诱导白血病细胞凋亡的作用,且小檗碱可增强柔红霉素的抗肿瘤作用,为其临床应用提供了依据。Objective To investigate the effect of berberine on inducing apoptosis of human T-lymphoblastic leukemia cell line (MOLT-4). Methods The exponentially growing MOLT-4 cells were divided into three groups: A, B and C. Group A were given 10 or 100 mg·L -1 of berberine hydrochloride, group B were given 1 mg L -1 of daunorubicin, and group C were given berberine hydrochloride plus daunorubicin. The cell survival rate were determined by MTT after 6 and 24 h incubation; the apoptosis were detected by flow cytometry, electrophoresis and microscopy. Results After incubation for 6 and 24 h, the cell survival rates in group A were 81.80% and 64.67%, group B were 98.38%and 38.46%, and group C were 68.35% and 20.51% . There was a significant difference in group C compared with group A and group B within either 6 or 24 h ( P <0.05). Berberine hydrochloride could induce the typical apoptosis changes in morphology changes and DNA ladder. The flow cytometry analysis showed that the apoptosis rates were 5.83% by 10 mg·L -1 berberine hydrochloride, and 19.93% by 100 mg·L -1 berberine hydrochloride at 24 h. Conclusion Berberine hydrochloride had apoptosis-inducing-effect on leukemia cells and could enhance the antileukaemia activities of daunorubicin, which might provide a theoretical basis for its clinical application.

关 键 词：小檗碱 白血病 细胞凋亡 

分 类 号：R965[医药卫生—药理学] R286[医药卫生—药学]