高效液相色谱法测定加替沙星眼凝胶中加替沙星的含量  

Determination of Gatifloxacin in Gatifloxacin Ophthalmic Gel by HPLC

作　　者：王晓玲[1] 李玉兰[2] 李东[1] 聂中越[1] 

机构地区：[1]广东省深圳市人民医院,暨南大学医学院第二附属医院药学部,518020 [2]广东省深圳市药品检验所,518029

出　　处：《医药导报》2005年第7期623-624,共2页Herald of Medicine

基　　金：广东省医学科学研究基金立项课题(基金编号:A2004605)

摘　　要：目的建立高效液相色谱法测定加替沙星眼凝胶中加替沙星含量.方法用Nucleosil 120-5 C18 (4.0 mm×250 mm,5 μm),以磷酸二氢钾缓冲液(磷酸二氢钾1 g溶解于纯化水1 000 mL,用磷酸调节pH值4.0)-甲醇(70 :30)为流动相,检测波长:286 nm,流速:1.0 mL·min-1,柱温:30℃.结果加替沙星在10～50 μg·mL-1浓度范围内呈良好的线性关系,平均回收率99.3%,RSD＝0.63%.结论该方法简便、快速、专属性强,可用于加替沙星眼凝胶中加替沙星的含量测定.Objective To establish an HPLC method for the determination of gatifloxacin in the gatifloxacin ophthalmic gel. Methods The stationary phase was Nucleosil 120-5 C_ 18 column (4.0 mm×250 mm, 5 μm) ,the mobile phase methanol-0.007 mol·L -1 KH_ 2 PO_ 4 buffer (pH adjusted to 4.0 with H_ 3 PO_ 4 ) (30∶70)with a flow rate of 1.0 mL·min -1 . The detect wavelength 286 nm ,and the column temperature 30℃. Results The calibration curve was linear within the range of 10-50 μg·mL -1 , the average recovery was 99.3%( RSD =0.63%, n =6). Conclusion This method is accurate and simple,it can be used for the determination of gatifloxacin in gatifloxacin ophthalmic gel .

关 键 词：加替沙星眼凝胶 高效液相色谱法 含量测定 

分 类 号：R978.69[医药卫生—药品] R927.2[医药卫生—药学]