3种分子标记分析油菜品种间的多态性效率比较  

Polymorphism analysis for maintainers and restorers of cytoplasmic male sterility in Brassica napus L.

作　　者：张书芬[1] 傅廷栋[1] 马朝芝[1] 朱家成[2] 王建平[2] 

机构地区：[1]华中农业大学遗传改良国家重点实验室,湖北武汉430070 [2]河南省农业科学院棉花油料研究所,河南郑州450002

出　　处：《中国油料作物学报》2005年第2期19-23,共5页Chinese Journal of Oil Crop Sciences

基　　金：国家"973"项目(2001CB10807);"十五"攻关(2001BA511B07);863项目(2002AA207009;2001AA241102);河南省杰出青年基金项目(0212000700)

摘　　要：应用RAPD、SSR和AFLP三种分子标记,对甘蓝型油菜恢复系垦C1和保持系1141B、32B的多态性进行了分析。在648条RAPD引物、196对SSR引物、414对AFLP引物中,两组合材料具有多态性的引物分别为172条和167条,131对和133对,147对和141对,而重复性好、多态性比率高的引物分别有78条,50对,120对组合;结果表明,平均每个(对)引物可以扩增出多态性片段数目为RAPD2.1个,SSR2.6个,AFLP引物12.7个。AFLP多态性检测效率高。AFLP、SSR是研究油菜遗传多样性比较有效的分子标记。RAPD, SSR and AFLP assay were performed in CMS restorer Ken C_1 and two maintainers 1141B and 32B in Brassica napus L.. Among 648 RAPD primers, 196 SSR primer pairs and 414 AFLP primer-enzyme combinations, 78 RAPD primers, 50 SSR primer pairs and 120 AFLP primer-enzyme combinations showed a high polymorphism ratio and a good repeatability between Ken C_1 and 1141B. The average number of polymorphic fragments generated by RAPD and SSR primers was 2.1 and 2.6, respectively, whereas that of AFLP primer-enzyme combinations was as high as 12.7. Based on these results, we conclude that high quality map can be obtained by AFLP and SSR molecular markers.

关 键 词：甘蓝型油菜 分子标记 RAPD SSR AFLP 多态性 

分 类 号：S565.403[农业科学—作物学]