植物雌激素α-ZAL对oxLDL诱导的人内皮细胞分泌NO和ET-1的影响及其机制研究  

Effect and mechanism of phytoestrogen α-Zearalanol on NO and ET-1 secretion induced by oxLDL in human umbilical vein endothelial cells

作　　者：徐海珊[1] 段金虹[1] 孙仁宇[1] 王小明[1] 许雪梅[1] 吴云清[1] 程锦轩[1] 戴顺龄[1] 

机构地区：[1]中国医学科学院基础医学研究所.中国协和医科大学基础医学院病理生理室,北京100005

出　　处：《中国药理学通报》2005年第6期692-697,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30170376)

摘　　要：目的探讨植物雌激素α-ZAL影响oxLDL诱导的人脐静脉内皮细胞分泌NO、ET-1的机制。方法采用RTPCR方法对eNOS、iNOS、ET-1mRNA的表达进行分析;采用WesternBlotting方法对eNOS、iNOS蛋白表达进行分析;同时测定内皮细胞上清液中NO、ET-1分泌的变化。结果植物雌激素α-ZAL增加oxLDL诱导内皮细胞eNOSmRNA、蛋白的表达和NO的分泌,降低ET-1mRNA的表达和蛋白的分泌。结论植物雌激素α-ZAL通过上调oxLDL诱导内皮细胞eNOSmRNA、蛋白的表达和NO的分泌,下调ET1mRNA的表达和蛋白的分泌起着保护血管内皮的作用。Aim To evaluate the effect of a novel phytoestrogen α-Zearalanol on oxLDL-induced effect on NO and ET-1 production in human umbilical vein endothelial cells.Methods Protein and mRNA expression of NOS and ET-1 were measured by Western blot and RT-PCR, levels of NO and ET-1 were measured by spectrophotometry and enzymatic immunoassay.Results Our results indicated that oxLDL significantly reduced NO release and enhanced ET-1 production associated with reducing eNOS protein/mRNA expression and enhancing ET-1 mRNA expression. α-Zearalanol could attenuate or abolish these oxLDL-induced alterations.Conclusion Phytoestrogen α-Zearalanol may effectively antagonize oxLDL-induced decrease in NO and increase in ET-1, which may be protective for endothelial function.

关 键 词：植物雌激素 氧化低密度脂蛋白 NO ET-1 

分 类 号：R977.1[医药卫生—药品]