镉、锌对人胚肾细胞的联合作用  

Combined Effect of Cadmium and Zinc on Human Embryo Nephridium 293 Cells

作　　者：董琛[1] 金泰廙[1] 雷玲[1] 常秀丽[1] 

机构地区：[1]复旦大学公共卫生学院 劳动卫生教研室,上海200032

出　　处：《环境与职业医学》2005年第3期193-196,共4页Journal of Environmental and Occupational Medicine

基　　金：国家973项目(编号:2002CB512905)

摘　　要：[目的]分析镉对肾细胞毒作用的剂量-效应关系和时间-效应关系,并观察锌对镉所致损伤的保护作用。[方法]实验采用人胚肾细胞(293细胞),设对照组、锌组、镉组、镉+锌组。噻唑蓝(MTT)和乳酸脱氢酶(LDH)测定各组对293细胞的毒作用;流式细胞仪分析细胞凋亡情况。[结果]实验发现20和40μmol/L镉作用于细胞48h,细胞相对存活率明显降低,细胞上清液中的LDH明显增高,并且在2.5-40μmol/L范围内存在明显的时间-效应、剂量-效应关系。氯化锌50μmol/L作用12h,细胞相对存活率、细胞上清液中的LDH以及细胞凋亡,与对照组比较均无明显改变,选择锌50μmol/L作为联合作用浓度。镉+锌组与单独加镉组相比细胞存活率增高,细胞上清液中的LDH和凋亡细胞比例减少。[结论]氯化镉对于293细胞膜有明显的损伤作用,可引起细胞膜通透性增加、细胞内LDH漏出增加、细胞凋亡增加,上述指标在一定范围内存在时间-效应和剂量-效应关系。50μmol/L锌对于细胞膜在一定程度上有保护作用,能拮抗镉对于肾细胞的损伤作用。[Objective] To examine the dose/time-effect relationship of cytotoxicity of cadmium to human embryo nephridium cell (293 cell) and observe the effect of co-exposure to cadmium and zinc. [Methods] The cell viability was measured by MTT assay, the activity of IDH was measured by colorimetry method and apoptosis of the cells was analyzed by flow cytometry. [Results] Treatment with different levels of CdCl2(20 μmol/L and 40 μmol/L) for 48 h, significantly reduced the viability and heighten the activity of IDH in 293 cells.Treatment with 50 μmol/L ZnCl2 for 12 h not significantly changed the viability and the activity of LDH,and apoptosis of cells could not be induced by ZnCl2 at concentration of 50 μmol/L. Treatment with zinc and cadmium, elevated cell viability and reduced apoptosis and activity of LDH compared with cadmium-exposure cells alone. [Conclusion] The direct toxicity of cadmium to 293 cells is likely related with the membrane damage. Treatment with cadmium induced apoptosis. The change of the indicators mentioned alove depended on time and dose. Zinc may have antagonistic joint action in 293 cells treated with cadmium.

关 键 词：镉 锌 联合毒作用 乳酸脱氢酶 细胞凋亡 

分 类 号：R135.1[医药卫生—劳动卫生]