外切酶保护PCR快速检测垃圾焚烧飞灰中二噁英类化合物  

The Determination of Dioxin-like Chemicals in Fly Ash Samples Using Exonuclease Protection Mediated-PCR Assay

作　　者：孙晞[1] 徐顺清[2] 

机构地区：[1]暨南大学食品系,广东广州510630 [2]华中科技大学同济医学院环境医学研究所,湖北武汉430030

出　　处：《环境与职业医学》2005年第3期202-204,共3页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金资助(编号:20107002;20377017)

摘　　要：[目的]利用外切酶保护PCR分析(exoucleaseprotectionmediated-PCRassay,EPM-PCR)方法检测焚烧炉飞灰中的二(?)英类化合物(dioxins-likechemicals,DLCs)的毒性当量(toxicequivalencyquantities,TEQs)。[方法]将两份5g飞灰样品经过抽提、浓缩及纯化等前处理步骤后,采用EPM-PCR方法检测其中的二(?)英类化合物的TEQs。与高分辨气相色谱-质谱联用技术(high-resolutionchromatographycombinedwithhigh-resolutionmassspectrometry,HRGC-HRMS)检测结果相比较,分析其差异原因。[结果]两份垃圾焚烧飞灰样品TEQs值分别为1288ng/kg和16000ng/kg,相当于根据HRGC-HRMS检测结果估算出来的TEQ值的1.61倍和2.63倍。EPM-PCR检测TEQ值偏高可能由于检测物中包含一些被化学方法排除的物质(如多氯联苯)造成。[结论]结合有效的前处理过程,EPM-PCR可快速地用于实际样品的定性与定量检测。[Objective] Exonuclease protection mediated-PCR(EPM-PCR) assay was applied to detect the toxic equivalency quantities(TEQs) of dioxin-like chemicals( DLCs) in fry ashes from incinerators. After the extraction, concentration and purification,5 g samples were analyzed by EPM-PCR assay. [Results] The TEQs of two samples were 1 288 ngTEQ/kg and 16 000 ngTEQ/kg respectively. Compared with those of the high-resolution chromatography combined with high-resolution mass spectrometry (HRGC/HRMS) method, the values were 1.61 and 2.63 times higher. The reason may be the presence of interference chemicals which are not detected by HRGC/HRMS,such as PCBs. [Conclusion] With the valid pretreatment process,EPM-PCR assay is valuable to screen and quantify the DLCs in samples rapidly.

关 键 词：二噁英类化合物 酶切保护PCR 毒性当量 高分辨气相色谱-质谱联用 

分 类 号：R122.12[医药卫生—环境卫生学]