小鼠卵母细胞和早期胚胎端粒酶活性的测定  

Telomerase activity in mouse oocyte and embryos during early development

作　　者：敬晓棋[1] 雷安民[1] 王晗[1] 杨学义[1] 窦忠英[1] 

机构地区：[1]西北农林科技大学国家干细胞工程技术研究中心陕西分中心,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2005年第6期27-30,34,共5页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家"863"高技术发展计划项目(2002AA216161);国家自然科学基金项目(302000137);国家教育部重大项目(03160)

摘　　要：　为了解小鼠早期发育过程中端粒酶的活性变化,本研究利用端粒重复序列扩增法(TRAP)进行了小鼠卵母细胞和附植前胚胎端粒酶活性的测定。结果表明,小鼠卵母细胞、桑椹胚和囊胚为端粒酶阳性,而2-细胞和8-细胞为阴性。依据电泳条带在成像系统下的光密度,计算相对总产品产量(TPG)的定量比较发现,囊胚端粒酶活性最高,为269.331;桑椹胚次之,为96.231;卵母细胞最低,为85.782。小鼠桑椹胚和囊胚胚胎记数及单细胞端粒酶活性比较结果显示,囊胚单细胞TPG最低,为3.45;桑椹胚单细胞略高于囊胚,为4.00;而卵母细胞最高,为85.782,大约是囊胚细胞的25倍。<Abstrcat>The telomerase activity in mouse oocyte and preimplantation embryo was examined using the polymerase chain reaction-based on telomeric repeat amplification protocol (TRAP).Telomerase activity was detected in mouse oocyte,morulae and blastocyst and not in 2-cells and 8-cells embryo.The quantitative comparison results showed,by ladder densitomitric methodology,that the total product generated (TPG) were 85.782,96.231,269.331 units in mouse oocyte,morulae and blastocyst respectively.However,the TPG of single cell of morulae and blastocyst were 4.00,3.45 units.So,the oocyte had the highest telomerase activity level about 25-fold of blastocyst.

关 键 词：小鼠 卵母细胞 早期胚胎 端粒酶活性 

分 类 号：Q132.7[生物学—普通生物学]