AcMNPV-1A在转P^(35)基因细胞系中连续传代的研究  

Serial passage of AcMNPV-1A in a transfected P^(35) gene cell line

作　　者：李守信[1] 李长友[2] 郑桂玲[2] 吴文君[1] 李国勋[2] 

机构地区：[1]西北农林科技大学农药研究所,陕西杨凌712100 [2]山东莱阳农学院害虫生防实验室,山东青岛266109

出　　处：《西北农林科技大学学报（自然科学版）》2005年第6期96-100,106,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(30370963)

摘　　要：　报道了1株粉纹夜蛾转P35基因工程细胞系,命名为Tn5B-35。苜蓿丫纹夜蛾核型多角体病毒(Au-tographacalifornicanuclearpolyhedrosisvirus,AcMNPV)在该细胞系中连续传代至25代,与原始细胞系Tn5B1-4相比较,其感染率、多角体产量、滴度以及杀虫毒力的变化趋势均比较平稳,并未出现"传代效应"。<Abstrcat>A new transfected P^(35) gene cell line,Tn5B-35,was derived by Trichoplusia ni Tn5B1-4 cell line.Autographa californica nuclear polyhedrosis virus (AcMNPV) was passaged in Tn5B-35 cells for 25 passages continuously and there was no significant change in morphology of the susceptibility,the changing trends of the occlusion bodies (OBs) yield,virus titer,and the larval corrected mortality after serial passage of the virus were stable,and there was no passage effect.

关 键 词：核型多角体病毒 昆虫细胞系 粉纹夜蛾 传代效应 

分 类 号：S476.13[农业科学—农业昆虫与害虫防治]