常通口服液对炎症因子参与大鼠肠粘连的影响  

Influences of Changtong Oral Liquid on Inflammatory Factors Joining Intestinal Adhesion in rats

作　　者：王春霞[1] 

机构地区：[1]南方医科大学南方医院药学部,广州510515

出　　处：《中国药师》2005年第7期535-537,共3页China Pharmacist

基　　金：国家重点科技攻关项目(NO:96-901-05-245)

摘　　要：目的:观察常通口服液对术后粘连大鼠血液中肿瘤坏死因子α(TNF-α),转化生长因子β1(TGF-β1),白细胞介素- 1β、4、6、10(IL-1β、IL-4、IL-6、IL-10)水平的影响。方法:选用SD雄性大鼠,随机分为6组:正常对照组、模型对照组、四磨汤组及常通口服液低、中、高剂量组。除正常对照组外,其余大鼠均制备成肠粘连模型。各组于术后第7天取血,酶联免疫吸附法(ELISA)测定细胞因子水平;同时用肉眼观察法进行粘连程度肉眼分级。结果:常通口服液能显著减轻模型大鼠的肠粘连程度,降低术后肠粘连大鼠血清中致炎细胞因子TNF-α、IL-1β、TGF-β1、IL-6水平。但对抗炎细胞因子IL-4、IL-10水平无影响。结论:本实验为术后粘连临床疗效评价指标筛选提供了实验依据。Objective: To observe the effect of Changtong oral liquid (CTOL) on the levels of TNF-α,TGF-β1 ,IL-1β,IL-4,IL-6 and IL-10 in the blood of rats after post operative intestinal adhesions. Method: Sprague-Dawley (SD) male rats were used in this study and randomly divided into six groups: normal controlled; model controlled, Simo Decoction and CTOL ( minimal, medium, and maximal dosages) . Except the normal controlled group, the other rats were made as intestinal adhesive models. On day 7 after surgery, the blood samples of each group were collected to determine the inflammation factor levels by ELISA; additionally, the levels of adhesion were graded by naked eyes. Result: CTOL could evidently reduce the severity of postoperative intestinal adhesions and decrease the serum levels of proinflammatory factors, such as TNF-α,IL-1β,TGF-β1 and IL-6. However, there was no significant impact on serum levels of anti-inflammatory factors, such as IL-4, IL-10 of postsurgery intestinal adhesive rats. Conclusion: This experiment had provided experimental basis for evaluation indexes of clinical therapeutic effects for postoperative adhesion.

关 键 词：常通口服液 肠粘连 细胞因子 

分 类 号：R285.5[医药卫生—中药学]