应用生物反应器体外构建组织工程化血管平滑肌层  

Constructing tissue engineering blood vessel in a bioreactor

作　　者：许志成[1] 李宏[1] 刘阳[1] 崔磊[1] 刘伟[1] 曹谊林[1] 

机构地区：[1]上海第二医科大学附属第九人民医院整形外科上海组织工程研究与开发中心,200011

出　　处：《中华实验外科杂志》2005年第7期807-809,i001,i002,共5页Chinese Journal of Experimental Surgery

基　　金：国家973重点基础研究项目(1999054309);国家863重点基础研究项目(2002AA205011)

摘　　要：目的探讨生物反应器内构建具有一定强度的组织工程化血管的可行性。方法将体外培养扩增的猪血管平滑肌细胞接种于聚羟基乙酸(PGA)上,形成细胞材料复合物,将其置于生物反应器内培养。实验组(n=5)为动态力学刺激培养(搏动频率:75次/分;扩张量:<5%);对照组(n=5)为静态培养。3周后行大体观察及组织学检测。结果血管样组织直径5mm,长10mm,厚1mm。实验组具有良好的弹性,管腔圆;平滑肌纤维与弹力纤维较多,排列有层次。对照组弹性欠佳,管腔塌陷;平滑肌纤维与弹力纤维较少且排列紊乱。结论应用生物反应器可构建具有良好弹性的血管平滑肌层组织。Objective To investigate the feasibility of in vitro constructing tissue engineered blood vessels in bioreactor.[WT5”HZ]Methods Cell-polyglycolic acid (PGA) complex was constructed by seeding vascular smooth muscle cells on PGA unwoven fibers,then cultured in a bioreactor (pulse rate=75/min,radical distension<5%).After three weeks in vitro culture,engineered tissues were examined both grossly and histologically.[WT5”HZ]Results Tissue engineered blood vessels with 5 mm in diameter,about 10 mm in length and 1 mm in thickness were harvested 3 weeks later.Grossly,the experimental groups showed a tubular structure with a round lumen and good elasticity.Histological staining revealed smooth muscle fibers layers and dense elastic fibers presented in the engineered vessel wall.In contrast,the control group took on poor elasticity and collapsed lumen.In addition,its arrangement of smooth muscle fibers and elastic fibers was random and disorganized.Conclusion Blood vessel with good elasticity can be constructed in a bioreactor using tissue engineering approach.

关 键 词：生物反应器 体外构建 组织工程化血管 平滑肌纤维 体外培养扩增 弹力纤维 聚羟基乙酸 血管平滑肌 组织学检测 细胞接种 搏动频率 力学刺激 静态培养 肌层组织 实验组 对照组 复合物 扩张量 弹性 管腔 排列 

分 类 号：R318.1[医药卫生—生物医学工程]