奥曲肽体外抑制血管生成的研究  

Octreotide inhibiting angiogenesis in vitro

作　　者：荚卫东[1] 王鲁[1] 许戈良[2] 

机构地区：[1]复旦大学中山医院肝癌研究所,上海200032 [2]安徽省立医院普外科

出　　处：《中华实验外科杂志》2005年第7期815-817,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目(30100077);全国优秀博士学位论文作者专项基金资助项目(200263)

摘　　要：目的探讨奥曲肽对人脐静脉内皮细胞(HUVECs)体外构建新生血管的抑制作用。方法应用噻唑蓝(MTT)实验、流式细胞仪、侵袭和迁移实验、Matrigel实验,研究不同浓度奥曲肽(1×10-10～1×10-5mol/L)对血管内皮生长因子(VEGF)诱导的HUVECs增殖、侵袭、迁移和分化成血管能力的影响。应用逆转录聚合酶链反应(RTPCR)检测HUVECs生长抑素受体(SSTR)的表达。结果奥曲肽浓度在1×10-8～1×10-5mol/L时抑制VEGF诱导的HUVECs增殖;流式细胞仪检测表明HUVECs被扣留在细胞周期G0/G1期;浓度为1×10-7～1×10-5mol/L时,奥曲肽抑制VEGF诱导的HUVECs侵袭作用,对HUVECs迁移无影响;浓度为1×10-8mol/L时,奥曲肽抑制HUVECs体外分化成管样结构,浓度为1×10-6mol/L时,完全阻断HUVECs体外分化成管样结构。RTPCR显示HUVECs表达高水平的SSTR3。结论奥曲肽对HUVECs体外构建新生血管具有抑制作用。Objective To investigate the inhibitory effect of octreotide on angiogenesis formed by human umbilical vein endothelial cells (HUVECs) in vitro.Methods Using MTT assay,flow cytometry,invasion assay,migration assay,and Matrigel assay,the effects of octreotide on the proliferation,invasion,migration and differentiation of HUVECs stimulated by VEGF were evaluated.The concentration of octreotide was from 1×10 -10 to 1×10 -5 mol/L.The expression of somatostatin receptor (SSTR) subtypes in HUVECs was detected by RT-PCR analysis.Results Octreotide inhibited proliferation of HUVECs stimulated by VEGF at the concentration of 1×10 -8 to 1×10 -5 mol/L.Flow cytometry analyses revealed that octreotide treatment arrested HUVECs at the G_0/G_1 phase of the cell cycle.Octreotide dose-dependently inhibited HUVECs invasion in response to VEGF.This inhibition was selective for invasion,as migration in the absence of the basement membrane barrier was not affected.Inhibition of tube formation was observed in the presence of 1×10 -8 mol/L octreotide and complete inhibition was obtained at 1×10 -6 mol/L octreotide.RT-PCR analysis demonstrated that HUVECs expressed SSTR3.Conclusion Octreotide dose-dependently inhibited angiogenesis formed by HUVECs in vitro.

关 键 词：抑制血管生成 血管内皮生长因子(VEGF) HUVECs 逆转录一聚合酶链反应 人脐静脉内皮细胞 MATRIGEL mol/L 流式细胞仪检测 生长抑素受体 RT-PCR 抑制作用 新生血管 体外构建 奥曲肽对 体外分化 moL/L 方法应用 不同浓度 

分 类 号：R965[医药卫生—药理学]