血管内皮生长因子在人类和鼠成骨细胞中的选择性剪接表达  

Alternative splicing expression of VEGF in osteoblasts of human and rat

作　　者：李长有[1] 宋今丹[2] 原银栋[1] 范广宇[1] 

机构地区：[1]中国医科大学附属第一医院骨科,沈阳110001 [2]中国医科大学基础医学院细胞生物学教研室卫生部重点实验室

出　　处：《中华实验外科杂志》2005年第7期835-836,共2页Chinese Journal of Experimental Surgery

摘　　要：目的探讨血管内皮生长因子(VEGF)各种转录本亚型在成骨细胞分化中的作用。方法应用特殊设计的引物,逆转录聚合酶链反应(RTPCR)检测VEGFmRNA在人类成骨细胞样细胞SaOS2和MG63和胎鼠颅盖骨细胞(FRC)在第0、5、9、14天不同分化阶段中的选择性剪接表达。结果人类成骨细胞样细胞SaOS2和MG63表达所有的VEGFmRNA亚型,其中以VEGF121和VEGF165表达最强。鼠FRC细胞表达3种VEGFmRNA亚型(即VEGF120,VEGF144和VEGF164),其中以VEGF120和VEGF164表达最强。伴随着FRC细胞的增殖、分化和矿化,这些亚型的表达逐渐增强,并且在结节矿化后达到最高水平。结论小分子的VEGF121/120和VEGF165/164在成骨细胞的分化过程中表达增强,可能起重要的血管源性调节作用。Objective To study the functions of various VEGF isoforms on osteoblast differentiation.Methods Using the specific primer sets,the expression of VEGF mRNA alternative splicing was detected in human osteoblast-like cells SaOS-2 and MG63 and in different differentiated rat FRC cells (on the day 0,5,9,14) by RT-PCR.Results[WT5”BZ] All 5 VEGF mRNA splice variants were expressed in human osteoblast-like cells SaOS-2 and MG63,and the smaller VEGF121 and VEGF165 variants were most abundant.Three VEGF mRNA splice variants,namely VEGF120,VEGF144,and VEGF164,wereexpressed in rat FRC cells,and the smaller VEGF120 and VEGF164 mRNA were most abundant.The expression intensity of these smaller variants was gradually increased during proliferation,differentiation and mineralization of FRC cells,and reached the highest level on the occurrence of mineralization.Conclusion The expression of smaller VEGF121/120 and VEGF165/164 was increased,and these variants might play an important angiogenesis role to modulate osteoblast differentiation.

关 键 词：选择性剪接 血管内皮生长因子(VEGF) 人类 成骨细胞样细胞 SAOS-2 VEGF165 成骨细胞分化 聚合酶链反应 颅盖骨细胞 方法应用 mRNA 细胞表达 表达增强 分化过程 调节作用 血管源性 A亚型 转录本 逆转录 FRC C细胞 高水平 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]