Genistin对Aβ_(1-42)诱发的PC12细胞损伤的神经保护作用  

The protective effects of Genistin on the PC12 cells injury induced by amyloid β peptide

作　　者：陈伟强[1] 张德兴[1] 罗利琼[2] 赵宇红[3] 杨红[1] 

机构地区：[1]广东药学院基础学院,广州510224 [2]广东药学院生物制药系 [3]广东药学院药科学院

出　　处：《解剖学研究》2005年第2期88-90,共3页Anatomy Research

基　　金：广东省中医药局资助项目(编号103079)

摘　　要：目的探讨Genistin对β淀粉样蛋白片段(Aβ1-42)诱导的PC12细胞损伤的神经保护作用。方法将培养的PC12细胞分为:正常对照组、Aβ1-42处理组和Genistin预处理组。Aβ1-42处理组用Aβ1-42处理细胞,Genistin预处理组在用Aβ1-42处理前2h加入Genistin。使用细胞形态学方法、LDH法和MTT法观察Genistin的神经保护作用。结果单纯Aβ1-4210滋mol/L处理细胞24h,MTT吸光值减小,LDH释放量明显增加,与正常对组比较差异有显著性(P<0.01);细胞形态发生明显改变。用25mmol/LGenistin预处理PC12细胞2h,MTT吸光值与Aβ1-42处理相比明显增加,LDH释放量显著降低,与Aβ1-42处理组相比差异有显著性意义(P<0.01)。结论Genistin在体外抑制Aβ1鄄42对细胞的毒性作用。Objective To investigate the effect of Genistin on the PC12 c ells' injury induced by beta-amyloid peptide fragment 1-42(Aβ1-42). Methods Th e cultured cells were divided into three groups: control group, Aβ1-42 group an d genistin pretreatment group. The cells in Aβ1-42 group were treated with Aβ1 -42, cells in pretreatment group were treated by Genistin, 2 hours later, it was treated by Aβ1-42. The effect of Genistin on PC12 cells' injury induced by Aβ 1-42 was measured or observed by using LDH release, MTT assays and cellular morp hology. Results Treatment of PC12 cells with Aβ1-42 (10 mmol/L) for 24 h caus ed a great decrease of MTT absorbance as well as the increase of LDH activities from PC12 cells. Compared with control group, there were great different between them (P<0.01). It can result in the change of cellular morphology. After the ce lls had been pretreated with 25 mmol/L Genistin for 2 h, MTT value of cells incr eased. The LDH value decreased. Compared with Aβ1-42 group, there were signifi cantly differentces between Aβ1-42 group and pretreatment group (P<0.01)). Con clusion Genistin could attenuate the cyotoxic effect induced by Aβ1-42 and th en it has pretective effect.

关 键 词：GENISTIN AΒ1-42 诱发 PC12细胞损伤 神经保护作用 阿尔茨海默病 

分 类 号：R285[医药卫生—中药学]