中药金叶败毒制剂抗人巨细胞病毒感染的作用机制  

Chinese Medicine Jinyebaidu on Human Cytomegalovirus Infection

作　　者：袁慧[1] 刘世新[2] 闻良珍[1] 李丹[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430030 [2]华中科技大学同济医学院法医系,武汉430030

出　　处：《华中科技大学学报（医学版）》2005年第3期345-347,352,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.30271673)

摘　　要：目的探讨中药金叶败毒制剂抗人巨细胞病毒(HCMV)感染的作用机制。方法用HCMVAD169感染人胚肺成纤维细胞,采用MTT法观察细胞的增殖活性,应用半定量RTPCR方法检测中药金叶败毒制剂和更昔洛韦(GCV)干预后感染细胞cyclinD1mRNA及细胞周期素依赖激酶4(CDK4)mRNA的表达水平。结果金叶败毒制剂和更昔洛韦能明显提高HCMV感染细胞cyclinD1及CDK4的表达,诱导G0/G1期细胞进入S和G2/M期,从而促进宿主细胞增殖。结论金叶败毒制剂可能通过促进HCMV感染细胞cyclinD1及CDK4的表达,促进宿主细胞增殖而发挥抗病毒作用。Objective To explore the action mechanism of Chinese medicine Jinyebaidu on human cytomegalovirus (HCMV) infection.Methods The cell proliferation activity was observed by MTT and the expression of the cyclinD1 mRNA and cell cycle dependent kinase 4 (CDK4) mRNA in the infected cells was detected by semi-quantitative RT-PCR before and after treatment by Jinyebaidu or Ganciclovir respectively.Results Both Jinyebaidu and Ganciclovir could increase the expression of cyclinD1 and CDK4 significantly in HCMV infected cells, induce and enhance cell proliferation from G_0/G_1 to S and G_2/M phases.Conclusion Jinyebaidu agent may play the antivirus action by increasing the expression of cyclinD_1 and CDK4 in HCMV infected cells and accelerating host cell proliferation.

关 键 词：金叶败毒制剂 巨细胞病毒 细胞周期素D1 细胞周期素依赖激酶4 细胞增殖 

分 类 号：R373.4[医药卫生—病原生物学]