双波长分光光度法测定氧氟沙星葡萄糖注射液中氧氟沙星的含量  

Determination of Ofloxacin in Glucose Injection by Dual-Wavelength Spectrophotometry

作　　者：周红娇 

机构地区：[1]云南省荣军康复医院,云南昆明650214

出　　处：《昆明理工大学学报（理工版）》2005年第3期100-102,共3页Journal of Kunming University of Science and Technology(Natural Science Edition)

摘　　要：应用双波长分光光度新计算法,以0.1mol/L盐酸为溶媒,选择293.0nm为测定波长,271.5nm为等吸收波长,不经分离,直接测定氧氟沙星葡萄糖注射液中氧氟沙星的含量,可消除葡萄糖的分解产物5-羟甲基糠醛(5-HMF)对氧氟沙星含量测定的干扰.方法简便,结果可靠.平均回收率为100.14%,RSD=0.38%.A new calculative method of dual-wavelength spectrophotometry is used for the determination of ofloxacin in ofloxacin glucose injection. The detection is performed at 293.0 nm as measurement wavelength and 271.5 nm as absorption wavelength, with 0.1mol/L HCl as resolvent. This simple method determines the contents of ofloxacin directly and accurately, since it does not need separation, which eliminates the interference caused by the decomposition product of glucose. The average recovery is 100.14%, RSD0.38%.

关 键 词：氧氟沙星注射液 双波长分光光度法 药物分析 

分 类 号：O657.3[理学—分析化学]