不饱和脂肪酸对花生四烯酸代谢产物的影响  

Effect of Polyunsaturated Fatty Acid on Metabolic Products of Arachidonic Acid

作　　者：董伟[1] 李新建[1] 陈泮藻[1] 郝秀华[1] 孟宪钧[1] 

机构地区：[1]中国人民解放军总医院临床基础医学研究所

出　　处：《标记免疫分析与临床》1994年第4期210-212,203,共4页Labeled Immunoassays and Clinical Medicine

摘　　要：本文用不饱和脂肪酸治疗脓毒症大鼠，观察治疗前、后大鼠血浆ＴＸＢ２、６－酮－ＰＧＦ１α、ＰＧＥ２及肝脏枯否氏细胞膜、红细胞膜花生四烯酸（ＡＡ）水平的变化，并探讨其机理。结果表明脓毒症大鼠血浆三种ＡＡ代谢产物水平明显高于正常组（Ｐ＜０．０１）；枯否氏细胞膜和红细胞膜的ＡＡ含量明显降低（Ｐ分别＜０．０１和＜０．０５）。经富含不饱和脂肪酸的鱼油治疗后，血浆ＴＸＢ２、ＰＧＥ２明显降低，两种细胞膜ＡＡ含量明显升高（Ｐ＜０．０１）。但血浆６－酮－ＰＧＦ１α仍处于高水平状态。提示在炎性反应时细胞膜ＡＡ发生明显变化，而肠道给予不饱和脂肪酸，可以改变膜ＡＡ含量，减少炎症介质的产生。Plasma thromboxane B2(TXB2), 6-keto-prostaglandin F1α (PGF1α) and prostaglandin E2 (PGE2) levels and arachidonic acid (AA) concentration of both Kupffer cells and red blood cell membrane were determined in septic rats before and after treatment with polyunsaturated fatty acids (PUFAs). The plasma concentrations of three metabolic products of AA in septic rats were higher than those of the controls (P<0. 01), while the AA contents of Kupffer cell and red blood cell membrane were markedly lowered (P < 0. 05 and 0. 02, respectively). After treatment with fish oil rich in polyunsaturated fatty scid, plasma TXB2 and PGE2 levels were significantly reduced and AA contents of both cell membranes increased (P<0. 01), whereas plasma 6-keto-PGF1α remained at high level. The results demonstrate that prominent changes of cell membrane AA occur in inflammation and enteral PUFAs therapy may alter the contents of cell membrane and reduce the production of inflammatory mediators.

关 键 词：脂肪酸类 不饱和 花生四烯酸类 脓毒症 大鼠 

分 类 号：R631.105[医药卫生—外科学]