大鼠附睾尾部的精子参数与灌服昆明山海棠乙醇提取物后的改变及其抗生育效果观察  

Changes in sperm parameters in the cauda epididymides and antifertility effect after treatment with TH5 in rats

作　　者：周激文 骆毅 潘汝能 刘黎闻 罗宁云 彭林 张宪民[1] 吴大刚[1] 

机构地区：[1]云南省计划生育科学研究所,中国科学院昆明植物研究所

出　　处：《生殖医学杂志》1994年第4期214-217,共4页Journal of Reproductive Medicine

基　　金：国家计划生育委员会;云南省科委

摘　　要：本文报告３７只Ｗｉｓｔａｒ成年大鼠附睾尾部的三项精子参数值及季节等因素对精子参数的影响，并与每日灌服昆明山海棠乙醇提取物ＴＨ５（１１６ｍｇ／ｋｇ）３０天后的２ｌ只大鼠的改变及生育力进行比较。结果表明：对照组大鼠左、右侧附睾尾部的三项精子参数值虽各不相等但无明显差异（Ｐ＞０．０５），与ＴＨ５组停药２０天后剖检的大鼠相比，上述各项精子参数值间有极显著差异（ｐ＜０．０１），且所有服药大鼠完全丧失生育力；对照组于４～６月剖检的大鼠其精子明显低于１～３和９～１２月剖检的大鼠；用于生殖研究的大鼠的体重＞４００ｇ或睾丸容积＞３．５ｍｌ、＜１．０ｍｌ均可对精子质量有不良影响；至于停服ＴＨ５７０天后剖检的大鼠，其精子参数值和生育力与对照组无异。This paper reports the changes of three sperm parameters of the cauda epididymides and fertility in 3 7 adult male Wistar rats,after treatment by garage with TH 5 of Tripterygium hypoglaucum(Levl.)Hutch.in ethanol extract in 2l rats(116mg.kg-1/d×30d).The studies showed that sperm parameters in the left and right sides of the cauda epididymides of the control rats were not significantly different(P>0.05),but were very significantly different fP<0.01) when compared to rats 20 days after discontinuation of treatment.All tested rats lost their fertility completely.In the control rats,the sperm motility of the cauda epididymis of rats killed in April～June were markedly lower than those of killed in other months.We found that the rats with body weight over 400g,with testis-volume above 3.5ml or un- der l.Oml,can affect the sperm quality of the cauda epididymis.However,the sperm parameters and fer- tility in rats 70 days after discontinuation of treatment with TH5 showed no difference from the controls.

关 键 词：精子 昆明山海棠 抗生育 乙醇提取物 

分 类 号：R169.41[医药卫生—公共卫生与预防医学] R979.21