内皮素－1对大鼠黄体细胞孕酮生成的影响  

Effect of endothelin-1 on progesterone production by rat luteal cells in vitro

作　　者：徐斯凡[1] 赵白鸽[1] 郑月慧[1] 王忠兴[1] 周寿康[1] 赵伟[1] 吴晓芸[1] 

机构地区：[1]江西医学院生理教研室,上海市计划生育科学研究所

出　　处：《生殖医学杂志》1994年第3期159-162,共4页Journal of Reproductive Medicine

基　　金：国家及江西省自然科学基金

摘　　要：我们采用黄体细胞孵育的方法，观察了内皮素－１对离体黄体细胞孕酮生成的影响。发现内皮素－１能抑制黄体细胞孕酮的基础分泌及人绒毛膜促性腺激素（ＨＣＧ）刺激下的孕酮分泌。内皮素－１加入孵育的黄体细胞悬液１５分钟后，即发生明显地抑制孕酮分泌作用（Ｐ＜０．０１）。人内皮素－１的抗血清能反转内皮素－１的上述作用。实验结果提示，内皮素－１可能为一种卵巢内调节肽，它通过抑制黄体细胞的孕酮生成。The effect of endothelin- 1 on progesterone production by incubated rat luteal cells wasstudied in the present investigation, Endothelin-1 could inhibit the basic progesteroneproduction(10-7mol / L, p<0.05) and the progesterone secretion induced by hCG(10-9mol / L,P<0.05)from rat luteal cells. The beginning time is 15 min.The effect of endothelin-1 could be prevented speciallyby endothelin-1 antiserum.This study shows that endothelin-1 combines with its special binding sites onthe luteal cells, which leads to the decrease of progesterone production and suggests that endothelin-l isan intra-ovarian regulatory factor and is involved in the process ofcorpus luteum involution.

关 键 词：内皮素 黄体 孕酮 

分 类 号：R335.5[医药卫生—人体生理学] Q516[医药卫生—基础医学]