罗布麻叶中总黄酮含量的测定  

Determination of the Content of Total Flavonoids in Apocynum Venetum L.

作　　者：范维刚[1] 解成喜[1] 李锋[1] 艾尔肯.艾米尔 

机构地区：[1]新疆大学理化测试中心,乌鲁木齐市830046

出　　处：《光谱实验室》2005年第3期464-466,共3页Chinese Journal of Spectroscopy Laboratory

摘　　要：以芦丁为对照品,NaNO2-AlCl3-NaOH体系为显色剂分光光度法测定罗布麻叶内的黄酮类物质。确定了提取总黄酮的适当溶剂、时间和显色剂用量。建立了芦丁浓度-吸光度关系的回归方程为A=0.145+7.9464C,r=0.9993。平均回收率为97.67%,相对标准偏差为0.52%。方法简单、可行、重现性好。The total flavoniods in Apocynum Venetum L. were determined by spectrophotometry with rutin standard and NaNO2-AlCl3-NaOH system. The maximum absorption of lutin standard is at 510nm. The regression equation is A = 0. 145 + 7. 9464C(r = 0. 9993) with the average recovery of 97. 67% and RSD of 0. 52%. The method is simple, reproducible and suitable for the determination of total flavones.

关 键 词：分光光度法 罗布麻叶 总黄酮 

分 类 号：O657.32[理学—分析化学]