套式PCR法检测HIV感染者血清中HCVRNA  

DETECTION OF HCV RNA IN SERA OF HIV INFECTIONS BY NESTED POLYMERASE CHAIN RE-ACTION

作　　者：丁列明[1] 章明太[1] 刘充洲 

机构地区：[1]浙江医科大学传染病研究所

出　　处：《肝脏病杂志》1994年第2期98-100,共3页

摘　　要：用套式ＰＣＲ法对２５例人类免疫缺陷病毒Ｉ型（ＨＩＶ－Ｉ）感染者和１７例对照组血清作了ＨＣＶ－ＲＮＡ检测。ＨＣＶＲＮＡ阳性者９例，其中４例有输血或接受过血制品史，３例为静脉药瘾者（ＩＶＤＵＳ），另２例与ＨＩＶ阳性患者有ＩＶＤＵＳ和性接触史，对照组无１例阳性。有输血／血制品和ＩＶＤＵＳ危险因素的患者的ＨＣＶＲＮＡ检测的阳性率最高（８０％和５０％）。在ＨＩＶ伴ＨＣＶ感染的全部病例中，仅２例曾有ＡＳＴ和ＡＬＴ轻度升高。本研究表明，这两种病毒伴随感染率之高是与其相同的传播途径危险因素有关，而未发现此两种病毒间可能相互影响的证据。Twenty-five patients infected with human immunodeficiency virus Type l(HIV) and seventeen controlsbave been tested for hepatItis C virus(RNA) in their sera by nested polymerase chain reaction. Nine patientswere found to be positive for HCV RNA Among the positive patients,four had a history of a blood transfu-sion or had used blood products, three were intravenous drug users(IVDU’s)and two had sexual (heterosexu-al)contact with HIV positive patients and INDU′s None in controls was positive The highest percentage ofpositive rates were found in patients with risk factors of blood transfusion/blood products (80 %)and INVU′S(50 % ) In all patients infected by HCV and HIV,only two had slight elevations of AST and ALT.The studyindicated that the high concurrent infection rate of the two virus occurs berause the viruses are implicated incommon risk factors for transmission, No evidence was found concerning any possible interaction between the

关 键 词：丙型肝炎病毒 套式PCR HIV 并发症 

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