乙二醇、丙二醇对胎儿卵巢冻存效果的影响  

Effects of Ethylene Glycol and Propylene Glycol on Freezing Fetal Ovaries

作　　者：李跃萍[1] 王燕蓉[1] 沈新生[1] 蔡玉芳[1] 赵承军[1] 周文献[1] 崔岫[1] 朱万平[1] 黑长春[1] 

机构地区：[1]宁夏医学院组织学与胚胎学教研室,银川750004

出　　处：《宁夏医学院学报》2005年第3期169-172,F003,共5页Journal of Ningxia Medical College

基　　金：国家自然科学基金资助项目(30360112)

摘　　要：目的比较1.5mol/L的乙二醇和丙二醇两种低温保护剂对胎儿卵巢组织的保存效果。方法采用“一步降温法”对24～28周水囊引产的胎儿卵巢进行冻存,将解冻后的胎儿卵巢组织移植于去势裸鼠的双肾被膜下,分别于移植后2、4及8周取材,通过电镜观察及移植后的组织学、受体鼠血清雌激素水平测定和阴道脱落细胞涂片判断组织细胞活性。结果乙二醇对卵泡形态结构的保存优于丙二醇,后者在卵巢的部分区域可见不可逆性的冷冻损伤;乙二醇组动情周期恢复率为33.3%,移植后动情周期开始天数为(15.00±5.0)d,而丙二醇组动情周期恢复率为16.7%,移植后动情周期开始天数(23.50±6.26)d。移植2周后乙二醇组与丙二醇组的移植物存活率分别为83.3%、66.7%,两组受体鼠血清雌激素水平之间的差异具有统计学意义(P<0.05),异种移植8周后两组移植物的存活率分别为58.3%、33.3%。结论以1.5mol/L的乙二醇作低温保护剂行快速冷冻效果优于1.5mol/L的丙二醇。Objective To compare the vitality and function of human feta l ovaries cryopreserved frozen-thawed with either ethylene glycol (EG) or propylene glyco l (PG). Methods Applying one-step cooling procedure to freeze fet al ovaries from 24 to 28 weeks old human fetus, after rapid thawing the ovaries tissue were partially transpla ntated into renal subcapsular area of ovariectomized nude mice. The mice were ki lled 2 weeks, 4 weeks and 8 weeks later respectively, ovary cell were taken out and fixed for electronic microscope morphometric analysis. Mice sera were colle cted and to tal serum estradiol was measured by RIA. The recovery of the oestrous cycle was observed carefully. Results The intact microstructure of foetus o vary tissue cryopreserved in ethylene glycol (EG) was superior to that of in pr opylene glycol (PG), while in the later group we could see the obvious cell damage in the part ial area of ovary. After xenotransplantation, the recovery percentage of oestrou s cycle in EG group was 33.3%. The recovery of oestrous cycle began on day 15.00 ±5.0, while in the PG group they were 16.7% and on day 23.50±6.26. After 2 wee ks xenotransplantation, the survival rate after transplantation in EG and PG gro up was 83.3%, 66.7%, r espectively. The two groups of recipient mouse showed significent difference in serum estrogen level (P<0.05). The survival rate after 8 weeks' heterogen eous transplantation in the two groups was 58.3%, 33.3%, respectively. Conclus ion Ethylene glycol is superior to propylene glycol in cryopreservatio n.

关 键 词：乙二醇 丙二醇 胎儿 卵巢 冻存 

分 类 号：R711.7[医药卫生—妇产科学]