玉米幼胚和成熟胚愈伤组织分化反应性比较  

A comparison on tissue culture ability between maize nature embryo and immature embryo

作　　者：曹俊梅[1] 窦秉德[2] 李生强[1] 陈莉[1] 罗玉明[2] 白光宏[1] 

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]淮阴师范学院生物系,江苏淮安223300

出　　处：《新疆农业大学学报》2005年第2期10-13,共4页Journal of Xinjiang Agricultural University

基　　金：江苏省教育厅高新技术项目(JH03-003)

摘　　要：以鲜食玉米为主的13种基因型的成熟胚和幼胚为材料,对它们的愈伤组织诱导,继代培养和分化进行了系统研究。结果表明:玉米的成熟胚和幼胚愈伤诱导率相关,但两种胚诱导出的愈伤组织状态明显不同,幼胚诱导出的愈伤组织状态普遍比成熟胚的好;两种胚的愈伤分化率差异明显,成熟胚愈伤组织最高分化率为2.5%,幼胚愈伤组织最高分化率可达83.3%。This paper deals with studies on initiation, maintenance and differentiation of callus from mature embryo and immature embryo of thirteen genotypes in corn. The results showed that the callus induced frequency were correlated between mature embryo and immature embryo,but the callus quality was obviously different. Calli obtained from immature embryo were generally batter than those of mature embryo. The differentiation frequency was widely different between the two kinds of callus, only 2.5% differentiation frequency could be obtained on mature embryo callus, and the differentiation frequency on immature embryo callus could be up to 83.3%.

关 键 词：玉米 成熟胚 幼胚 组织培养 愈伤组织 组织分化 

分 类 号：S513[农业科学—作物学]