渗透胁迫对新疆大果沙枣幼苗叶片膜脂过氧化及膜保护酶的影响  

Effect of osmotic stress on leaf membrane lipid peroxidation and protective enzymes in Elaeagnus angustifolia L. seedlings

作　　者：李阳[1] 齐曼.尤努斯 安萍[2] 

机构地区：[1]新疆农业大学园艺学院,乌鲁木齐830052 [2]日本鸟取大学干旱地研究中心,日本6800001

出　　处：《新疆农业大学学报》2005年第2期47-50,共4页Journal of Xinjiang Agricultural University

摘　　要：采用PEG6000及NaCl进行渗透胁迫,研究渗透胁迫对新疆大果沙枣幼苗叶片膜脂过氧化及保护酶的影响。结果表明:随着渗透胁迫程度加大和胁迫时间的延长,新疆大果沙枣叶片中相对含水量逐步下降,叶片膜脂过氧化程度加重,丙二醛含量持续上升,交叉处理减缓了水分胁迫的伤害程度;交叉处理和重度渗透胁迫的超氧化物歧化酶、过氧化氢酶活性在处理16h前逐渐增加,16h后下降,轻度渗透胁迫超氧化物歧化酶活性在处理24h达到峰值,过氧化氢酶活性在整个处理过程中则保持上升趋势;各处理过氧化物酶活性变化趋势较一致,在处理16h达到其峰值,20%PEG6000处理32h左右能使新疆大果沙枣幼苗严重失水萎蔫。渗透胁迫明显影响新疆大果沙枣幼苗叶片膜脂过氧化程度的加重和保护酶活性的变化,新疆大果沙枣幼苗具有有限的耐渗透的能力。The effects of water and salt stress on leaf membrane liquid peroxidation and endogenous protective enzymes in Elaeagnus angustifolia L. seedlings, which were treated with the osmotic stress solution PEG6000 and NaCl, were studied .The results showed that as the time went on and the stress gradually increased,relative water content descended gradually, leaf membrane liquid peroxidation ascended,the malondialdehyde content increased evidently, the cross treatment reduced the degree of water stress. SOD and H2O2 activities of the cross treatment and high osmotic-stress ascended gradually before 16 hours, then descended, SOD activities of the gentle osmotic-stress reached its peak, and all the course H2O2 activities kept ascending. POD activities of three treatments changed uniformly and reached its peak. The treatment with 20%PEG6000 about 32 hours made the plant lose its water and droop down. This progress improved the osmotic stress and affected leaf membrane liquid peroxidation and endogenous protective systems, the ability of its osmotic tolerance was limited.

关 键 词：渗透胁迫 新疆大果沙枣 膜脂过氧化 酶 

分 类 号：S793.901[农业科学—林木遗传育种]