冠心病痰瘀证候与载脂蛋白E第一内含子增强子基因多态性关系的研究  

Study on Relationship between the Polymorphisms of Apolipoprotein E gene Intron 1 Enhancer element and The Phelgm and Blood stasis syndrome in Patients with Coronary Heart Disease

作　　者：欧阳涛[1] 宋剑南[1] 李林[2] 牛晓红[1] 金红[1] 陈冰[1] 苗阳[3] 林谦[4] 

机构地区：[1]中国中医研究院基础理论研究所,北京100700 [2]北京大学数学科学学院,北京100871 [3]中国中医研究院西苑医院,北京100091 [4]北京中医药大学东方医院,北京100078

出　　处：《中国中医基础医学杂志》2005年第6期414-417,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目(30171128);国家重点基础研究发展计划(2003CB517105)

摘　　要：目的:探讨冠心病(CHD)不同的痰瘀证候与载脂蛋白E基因第一内含子增强子(ApoEIE1)多态性的关系。方法:选择符合条件的CHD中医痰证、瘀证、痰瘀互阻证和非痰非瘀证(其他证型)患者200例,另选100例健康对照,常规检测所有样品的血脂水平。提取全血DNA,用聚合酶链式反应限制性片段长度多态性(PCRRFLP)技术检测apoEIE1的基因型。采用SAS软件进行相关性分析。结果:各基因型间血脂水平的差异未达到显著性意义,但CC,CG,GG基因型患者的总胆固醇(TC)、总甘油三酯(TG)和低密度脂蛋白胆固醇(LDLC)水平顺序有依次升高的趋势。CHD患者中的GG基因型显著多于健康对照,并在痰证和痰瘀互阻证中的分布多于瘀证和非痰非瘀证。G等位基因与apoE第四外显子ε4等位基因密切相关。结论:ApoE第一内含子增强子BspLI位点的GG基因型可能是冠心病痰证及痰瘀互阻证的易感基因型之一。Objective:Aim is to explore the relationship between the polymorphism of apoE exon 4 intron 1 enhancer element gene(apoE IE1)and the phlegm syndrome(PS)and blood stasis syndrome(BSS)differentiation-type(SDT)in patients with CHD.Methods:Differentiated by Syndrome Differentiation-type standards(TCM-SDT),200 CHD patients involved PS,BSS,Phlegm-Blood Turbid syndrome(PBTS),Non-Phlegm-Non-Blood stasis syndrome(NPNBS)were adopted.The controls are 100 healthy cases.Blood lipids were measured by routine examine.The peripheral blood total DNA were extracted.The apoE IE1 genotypes were determined by polymerase chain reaction -Restriction fragment length polymorphism(PCR-RFLP)technique.The relativity of data was analyzed by SAS software.Results:Occurrence of G/G genotype in CHD is much significantly higher than those in healthy control.Although the levels of cholesterol in genotypes couldn't appear significantly difference among them,there was still a gene-dosage effect on step-up TC,TG and LDL-C levels from C/C,G/C to G/G.The G/G genotypes in PBTS were significantly more than those in BSS and NPNBS.G allele was closely linkage with exon4 ε4 allele.Conclusion:ApoE IE1 BspLI site G/G genotypes may be a susceptible genotype to CHD PS and PBTS.

关 键 词：冠心病 APOE 增强子 基因多态性 痰瘀证候 

分 类 号：R216[医药卫生—中医学]