新型钙增敏剂MCI-154对失血性休克大鼠血管反应性的影响  

Effects of a new calcium sensitizer MCI-154 on vasoreactivity of hemorrhagic shock rats

作　　者：杨光明[1] 刘良明[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所第二研究室,创伤,烧伤与复合伤国家重点实验室,重庆400042

出　　处：《创伤外科杂志》2005年第2期118-122,共5页Journal of Traumatic Surgery

基　　金：国家自然科学基金资助项目 (30 2 712 6 6和 30 370 5 6 3);国家重点基础发展计划 (973) (G19990 5 4 2 0 2 )资助项目

摘　　要：目的　探讨钙增敏剂MCI 15 4对失血性休克大鼠血管反应性的影响。方法　实验分 2部分 :在体实验 ,4 2只Wistar大鼠随机分为正常组、休克组、MCI 15 4 (0 .1、0 .5、1.0、2 .0mg/kg)组 ,观察大鼠失血性休克后 ,MCI 15 4对去甲肾上腺素 (NE)升压反应的影响 ;离体实验 ,4 8只大鼠分为正常组、休克组、MCI 15 4(10 -7、10 -6、10 -5、10 -4mol/L)组 ,取失血性休克大鼠肠系膜上动脉 ,利用离体血管环张力测定技术观察MCI 15 4对NE诱导失血性休克大鼠肠系膜上动脉收缩反应性的影响。结果　在体实验 ,大鼠失血性休克 2小时后 ,NE的升压作用显著下降 ,给MCI 15 4后 ,NE的升压反应进一步降低 ,低于正常对照组和休克对照组。离体实验 ,与正常对照组相比 ,失血休克后血管环对NE的收缩反应性显著降低 ;不同浓度的MCI 15 4预孵 30分钟可使血管环的反应性进一步降低 (P <0 .0 1) ,且有剂量依赖性。结论　MCI 15 4C能降低失血性休克大鼠全身或局部血管的反应性。Objective To observe the effects of MCI-154 on vascular reactivity following hemorrhagic shock(HS) in rats.Methods The experiments were conducted in vivo and in vitro.In vivo,42 Wistar rats were randomly divide into 6 groups:normol control,hemorrhagic shock control and shock with MCI-154(0.1、0.5、1.0、2.0mg/kg).The hemorrhagic shock(30mmHg for 2 hours) model of rat was adopted to observe the effect of MCI-154 on pressor effect of norepinephrine(NE) in vivo.In vitro,48 rats were divide into 6 groups:normol control,hemorrhagic shock control and shock with MCI-154(10 -7 、10 -6 、10 -5 、10 -4 mol/L) groups.Superior mesenteric artery(SMA) was used to observe the effect of MCI-154 on NE induced-vascular contraction with isolated organ perfusion system.Results In vivo,the NE-induced pressor response following hemorrhagic shock(2 hours) was significantly(P<0.01) decreased.After pretreatment with MCI-154,the pressor response of NE was further decreased,it was significantly lower than that of the normal and shock control group.In vitro,the contractile response of SMA to NE following hemorrhagic shock was significantly reduced as compared with the control group.After MCI-154 pretreatment for 30 minutes,the responsiveness of SMA to NE was further decreased as compared to shock group(P<0.01),this effect of MCI-154 was in dose-dependent manner.Conclusion MCI-154 can reduce the vascular reactivity of hemorrhagic shock rats including the systemic responsiveness and local vascular reactivity.

关 键 词：出血 休克 钙增敏剂 血管反应性 

分 类 号：R605.971[医药卫生—急诊医学]