艾斯能对慢性低灌注大鼠认知功能的影响  

Effect of Exelon on cognitive function of chronic cerebral hypoperfusion rats

作　　者：孙麓 黄敬[1] 梅元武[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院神经内科

出　　处：《临床神经病学杂志》2005年第3期207-210,共4页Journal of Clinical Neurology

摘　　要：目的探讨艾斯能对慢性低灌注大鼠认知功能的影响及其作用机制。方法将112只大鼠随机分为正常组、低灌注组、盐水组、艾斯能组。除正常组外,其他3组大鼠予双侧颈总动脉结扎制模。4周后艾斯能组应用艾斯能治疗,盐水组予等量生理盐水,低灌注组及正常组常规喂养。6周后进行水迷宫试验、事件相关电位(ERP)测定、胆碱酯酶(AchE)浓度及胆碱(Ach)纤维密度测定,并进行组间比较。结果与正常组相比,低灌注组和盐水组水迷宫完成时间显著延长,错误次数显著增多,ERP潜伏期(PL)显著延长,AchE浓度及Ach纤维密度也显著下降(均P<0.05);艾斯能组AchE浓度及Ach纤维密度较低灌注组和盐水组进一步下降,但水迷宫试验结果较低灌注组和盐水组显著好转,ERP各波的PL均显著低于低灌组和盐水组(均P<0.05);低灌组和盐水组水迷宫试验结果、ERP结果、AchE浓度及Ach纤维密度无差异。结论艾斯能对慢性低灌注造成的大鼠认知功能损害有改善作用,可能与其抑制AchE、提高脑组织中Ach浓度有关。Objective To investigate the effect of Exelon on cognitive function of chronic cerebral hypoperfusion rats and its cholinergic mechanism.Methods 112 rats were randomly separated into four groups (normal, hypoperfusion, normal saline and Exelon). Bilateral common carotid arteries were ligated for models of cerebral hypoperfusion in the rats except normal group. 4 weeks later, Exelon and normal saline were used to treat the rats in Exelon group and normal saline group respectively for 6 weeks. Then the values of AchE lever and Ach fiber density together with the results of experiments in water maze and the values of event-related potential (ERP) were recorded and compared with each group.Results Comparing with the normal group, the water maze completion time of the hypoperfusion group was significantly delayed, error frequency significantly increased, the latency of ERP much extended, the AchE level and Ach fiber density evidently decreased(all P< 0.05). Comparing with the hypoperfusion group, the AchE level and Ach fiber density in Exelon group were much lower, but the water maze results was better and the potential latency of ERP was shorter(all P< 0.05).Conclusions Exelon has positive effect on prevention and improving cognitive impairment caused by chronic cerebral hypoperfusion. Inhibiting AchE and increasing acetylcholine level in brain may be involved in its mechanism.

关 键 词：脑低灌注 认知功能 事件相关电位 胆碱酯酶 艾斯能 

分 类 号：R96[医药卫生—药理学]