秤锤树离体培养和植株再生  

In Vitro Culture and Plant Regeneration of Sinojackia xylocarpa Hu

作　　者：蒋泽平[1] 梁珍海[1] 刘根林[1] 吴纲[1] 

机构地区：[1]江苏省林业科学研究院,南京211153

出　　处：《园艺学报》2005年第3期537-540,共4页Acta Horticulturae Sinica

基　　金：江苏省科技厅社会公益与服务专项资金资助项目(BM2001701)

摘　　要：在经选育的5年生秤锤树优良母株上,取其腋芽茎段和顶芽为外植体,进行离体培养和植株再生研究。结果表明,以改良MS+BA1.0mg/L+TDZ0.05mg/L+IBA0.05mg/L+维生素C50mg/L+3%蔗糖最适宜芽苗增殖;改良MS+BA0.5mg/L+TDZ0.05mg/L+IBA0.1mg/L+维生素C50mg/L+3%蔗糖最适宜芽苗壮苗生长;最适宜生根培养基为1/2MS+IBA0.5mg/L+NAA0.3mg/L+维生素C100mg/L+2%蔗糖;经生根的试管苗移栽于蛭石∶珍珠岩∶泥炭=6∶3∶1的混合基质,控制温度20～30℃,相对湿度85%～90%,保湿15～20d,其间适当遮荫,35d后成活率达92%以上。In order to protect and utilize effectively the endangered species Sinojackia xylocarpa Hu,orthogonal tests were designed to get the effects of plant hormones on the multiplication,growth and rooting of the species tube-shoots.A study was conducted on in vitro culture and plant regeneration of Sinojackia xylocarpa Hu with the nodal segments or apical buds excised from the newly-grown shoots of the five-year-old elite maternal plant.Results showed that the modified MS supplemented with BA 1.0 mg/L+TDZ 0.05 mg/L+IBA 0.05 mg/L+Vitamin C 50 mg/L+3% Sucrose was optimal for the tube-shoot multiplication while the modified MS with BA 0.5 mg/L+TDZ 0.05 mg/L+IBA 0.1 mg/L+Vitamin C 50 mg/L+3% Sucrose was suitable for the vigorous growth.1/2MS with IBA 0.5 mg/L+NAA 0.3 mg/L+Vitamin C 100 mg/L+2% Sucrose optimized the tube-shoot rooting induction. The plantlets with root were transplanted into the substrate containing vermiculate, pearlite and peat, 6∶3∶1(v/v). At 20-30℃,relative humidity of 85%-90% for 15-20 days,the plantlets could grow well,with the survival up to 92% after 35 days’transplanting.

关 键 词：秤锤树 离体培养 植物生长调节剂 维生素C 

分 类 号：S686[农业科学—观赏园艺]