氯锌油软膏中氯霉素含量的测定  

Determination of chloramphenicol in Lv Xin You ointment

作　　者：杨少芳[1] 曾庆凯[1] 张赤[1] 

机构地区：[1]武汉市儿童医院药学部,武汉430016

出　　处：《中国临床药学杂志》2005年第1期45-46,共2页Chinese Journal of Clinical Pharmacy

摘　　要：目的　建立测定氯锌油软膏中氯霉素含量的HPLC法。方法　先用石油醚溶解软膏中的小麻油,离心后弃去石油醚层,再加甲醇旋涡振荡提取软膏中的氯霉素。采用HypersiLC18柱(2 5 .0mm×4 . 0mm ,5 μm) ,流动相为甲醇-水(5 0∶5 0 ,V/V) ,流速1mL·min-1,进样量2 0 μL ;检测波长2 78nm ,柱温30℃,峰面积外标法计算。结果　氯霉素浓度在2 5～2 5 0mg·L-1范围内线性关系良好(r=0 . 9998) ,平均回收率为99 .7% ,RSD =0 . 89% (n =5 )。结论　本方法简便、准确,可用于本制剂的含量测定和质控。AIM To establish a HPLC method to determine chloramphenicol in Lv Xin You ointment by HPLC.METHODS A Hypersil (250 mm×4.0 mm,5 μm) column was used with a mobile phase consisted of amixture of methanol-water (50∶50,V/V) at a flow rate of 1 mL·min -1and UV detector wavelength at 278 nm.Column temperature was 30℃.RESULTS The average recovery of chloramphenicol (n=5) was 99.7%.The linear range of chloramphenicol was 25-250 mg·L -1(r=0.999 8).CONCLUSION This method is simple,accurate and can be used to control the quality of Lv Xin You ointment.

关 键 词：高效液相色谱 氯锌油软膏 氯霉素 

分 类 号：R927.2[医药卫生—药学]