HPLC-电化学法测定人血浆中盐酸克仑特罗浓度  

HPLC-electrochemical detection method for the determination of clenbuterol hydrochloride in human plasma

作　　者：郭红云[1] 路成吉[1] 

机构地区：[1]新乡医学院第三附属医院药剂科,新乡453003

出　　处：《中国临床药学杂志》2005年第2期117-119,共3页Chinese Journal of Clinical Pharmacy

摘　　要：目的　应用HPLC -电化学法检测人血浆中盐酸克仑特罗含量。方法　采用内标法定量,使用NucleodurC18色谱柱,以磷酸二氢钾缓冲液-乙腈(7∶3,V/V ,pH 5 .0 )为流动相,检测器电位0 .80V(Ag/AgCl)。结果　该方法的线性范围1.5～12 0μg·L-1,回归方程Y =0 .75 3X +0 .0 11,r=0 .9993;最低检测浓度0 .0 15 μg·L-1;RSD 2 .6 %～4 .2 % ;平均回收率93.4 %。AIM To develop a HPLC-electrochemical detection method to determine clenbuterol-hydrochloride in human plasma. METHODS The assay was based on internal standard with hydroquinone.The Nucleodur C 18 column was used. The mobile phase was phosphate buffer and acetonitrile (7∶3,V/V,pH 5.0).The potential of working electic was maintained +0.80V(Ag/AgCl). RESULTS The linear range was from 1.5-120 μg·L -1.The liner regression equation was Y=0.753X+0.011 (r=0.999 3). The minimal detection limit was 0.015 μg·L -1. The average recovery was 93.4% with RSD of 2.6%-4.2%.CONCLUSION This method is suitable for clinical use.

关 键 词：高效液相色谱法 电化学检测器 血浆 盐酸克仑特罗 

分 类 号：R96[医药卫生—药理学]