HPLC法测定帕金森病大鼠模型中鱼藤酮血浆浓度  

HPLC determination of rotenone in Parkinson disease's model rat plasma

作　　者：黄君[1] 裴元英[1] 

机构地区：[1]复旦大学药学院药剂学教研室,上海200032

出　　处：《中国临床药学杂志》2005年第3期161-163,共3页Chinese Journal of Clinical Pharmacy

摘　　要：目的建立测定帕金森病模型大鼠血浆中鱼藤酮浓度的HPLC方法。方法大鼠血浆经预处理后,采用反相HPLC法紫外检测器检测。色谱柱为DikmaDiamonsilC18柱(200mm×4.6mm,5μm),保护柱为WatersNovaParkC18柱,流动相∶乙腈-水=55∶45(V/V),流速1mL·min-1,检测波长(λ)210nm。结果本方法测定鱼藤酮在25～1600μg·L-1范围内线性良好(r=0.9999,n=7),最低检测浓度为5μg·L-1。高、中、低浓度回收率均>90%,日内、日间RSD在0.28%～1.92%。结论本方法适用于大鼠血浆鱼藤酮浓度的检测。AIM To establish a simple, sensitive and rapid HPLC method for determination of rotenone in Parkinson disease's rat plasma. METHODS The rotenone in plasma samples was extracted with ethyl acetate . The separation used a reversed-phase Dikma Diamonsil C 18 column (200 mm×4.6 mm,5 μm),guard column Waters Nova-Park C 18 column,with mobile phase consisting of acetonitrile-water (55∶45, V/V), the flow rate was 1 mL·min -1,the detective wavelength was 210 nm. RESULTS The linear range was 25-1 600 μg·L -1(r=0.999 9,n=7), the limit of detection for rotenone in plasma was 5 μg·L -1.All the recovery of low, medium and high concentrations were above 90% and the relative standard deviations of intra-day and inter-day assay were within 0.28%-1.92%. CONCLUSION This method provides an analytical procedure for the determination of rotenone concentration in plasma.

关 键 词：鱼藤酮 帕金森病 高效液相色谱 

分 类 号：R285[医药卫生—中药学]