4种检测耐甲氧西林金黄色葡萄球菌方法的评估  

Evaluation of four techniques for in detection of methicillin-resistant staphylococcus aureus

作　　者：刘瑛[1] 张蓓[1] 沈立松[1] 

机构地区：[1]上海第二医科大学附属新华医院检验科,上海200092

出　　处：《诊断学理论与实践》2005年第3期225-227,共3页Journal of Diagnostics Concepts & Practice

摘　　要：目的:评价4种检测耐甲氧西林金黄色葡萄球菌(MRSA)方法的临床应用价值。方法:用mecA基因检测MRSA乳胶凝集法、头孢西丁纸片扩散法和苯唑西林微量稀释法对临床分离的160株金黄色葡萄球菌进行检测并作比较。结果:160株中有127株为MRSA,4种检测方法结果一致。结论:头孢西丁纸片扩散法可作为医院微生物实验室日常工作中检测MRSA的简便又准确的实验方法,并值得推广应用。Objective To evaluate the clinical application of the four methods for detection of methicillin-resistant staphylococcus aureus(MRSA). Methods PCR amplification of the mecA gene, the MRSA-screen latex agglutination test, disk diffusion method with cefoxitin and the microdilution method of oxacillin were performed to analyze 160 clinical isolates of S. aureus. Results One hundred and twenty seven strains were MRSA. The results from four methods were consistent. Conclusions Disk diffusion method with cefoxitin is simple and reliable for routine detection and confirmation of MRSA, which is suitable for wide application in the clinical laboratories.

关 键 词：耐甲氧西林金黄色葡萄球菌 MECA基因检测 MRSA乳胶凝集法 头孢西丁纸片扩散法 微量稀释法 

分 类 号：R378.11[医药卫生—病原生物学]