Expression of PHGPx in mammalian cells and its antiviral effect against coxsackievirus group B  

作　　者：ZHAOWenran ZHONGXuekuan ZHOULingwang 

机构地区：[1]InstituteofKeshanDisease,ChineseResearchCenterforEndemicDiseaseControl,HarbinMedicalUniversity,Harbin150086,China

出　　处：《Progress in Natural Science:Materials International》2005年第7期665-668,共4页自然科学进展·国际材料（英文版）

基　　金：Supported by the National Natural Science Foundation of China (Grant No. 30170803)partially supported by the Key Technologies Program of Heilongjiang Province (Grant No. GB01C10601)

摘　　要：To express human phospholipid hydroperoxide glutathione peroxidase (PHGPx) in eukaryotic cells and to study its antiviral effect against Coxsackievirus B3m (CVB3m) in vitro, PHGPx cDNA was amplified from a human testis library using specific primers and cloned into expression vector pcDNA3. 1/His. Expression of PHGPx was performed in COS-1 cells. The antiviral effect was studied by the treatment of HeLa cells with the recombinant PHGPx. Results showed that the activity of PHGPx expressed in COS-1 cells was 5-fold higher than that in control group, and it inhibited the cytopathic effect on HeLa cells caused by CVB3m. It can be concluded that recombinant PHGPx expressed in COS-1 cells has antiviral effect against CVB3m in vitro.To express human phospholipid hydroperoxide glutathione peroxidase (PHGPx) in eukaryotic cells and to study its antiviral effect against Coxsackievirus B3m (CVB3m) in vitro, PHGPx cDNA was amplified from a human testis library using specific primers and cloned into expression vector pcDNA3.1/His. Expression of PHGPx was performed in COS-1 cells. The antiviral effect was studied by the treatment of HeLa cells with the recombinant PHGPx. Results showed that the activity of PHGPx expressed in COS-1 cells was 5-fold higher than that in control group, and it inhibited the cytopathic effect on HeLa cells caused by CVB3m. It can be concluded that recombinant PHGPx expressed in COS-1 ceils has antiviral effect against CVB3m in vitro.

关 键 词：PHGPX gene expression coxsackievirus group B ntiviral effect 

分 类 号：Q78[生物学—分子生物学]