亚甲基四氢叶酸还原酶677C→T及1298A→C基因多态性与糖尿病心血管病变的相关性  

The relationship between N5,10-methylenetetrahydrofolate reductase (MTHFR) gene polymorphism and diabetic cardiovascular disease

作　　者：刘德敏[1] 范新萍[1] 于德民[1] 

机构地区：[1]天津医科大学代谢病医院实验室,300070

出　　处：《中华糖尿病杂志（1006-6187）》2005年第3期219-222,共4页

基　　金：天津市高等学校科技发展基金资助项目(991017)

摘　　要：目的探讨亚甲基四氢叶酸还原酶(MTHFR)基因多态性与糖尿病心血管并发症(DC)的关系。方法应用PCRRFLP法检测84例健康人(NC)和158例2型糖尿病患者[分为无DC(NDC)和DC组]的MTHFR677C→T及1298A→C基因多态性,比较各组间基因型分布和等位基因频率。结果DC组MTHFR677TT基因型分布及T等位基因频率均高于NC或NDC组,且叶酸水平较低(P<0.001);各组间MTHFR1298A→C基因型分布及等位基因频率差异无统计学意义(P>0.05);MTHFR基因型、年龄是DC的危险因子。结论MTHFR677C→T突变与DC的发生有关。Objective To explore the relationship between MTHFR gene polymorphism and ~diabetic cardiovascular disease. Methods The MTHFR C677T and MTHFR A1298C gene polymorphisms were detectd by PCR-RFLP to compare MTHFR C677T and MTHFR A1298C genotype distribution and allele frequencies among three groups. Enrolled were 84 healthy individuals (NC) and 158 T2DM subjects who were classified into two groups, i.e. non-diabetes complications (NDC) and ~diabetic cardiovascular disease (DC). Results Patients with diabetic cardiovascular disease had higher frequencies of MTHFR C677T mutation genotype and T allele than control and non-diabetes complications subjects, and had lower folate levels (P<0.001). There were no significant differences in MTHFR A1298C genotype distribution and allele frequencies among three groups (P>0.05). A logistic regression analysis demonstrated that MTHFR C677T and age were the independent predictors of DC. Conclution There is a strong correlation between the polymorphism of MTHFR C677T and diabetic cardiovascular disease.

关 键 词：亚甲基四氢叶酸还原酶 677C→T 1298A→C 基因多态性 糖尿病 心血管疾病 基因型 

分 类 号：R587.1[医药卫生—内分泌]