猪供心不同时间热缺血移植后细胞凋亡的变化  

The Expression of Myocardial cell Apoptosis on Different Warm Ischemic Heart Allograft Transplantation in Pigs

作　　者：孙忠东[1] 池一凡[1] 杨铁南[1] 侯文明[1] 牛兆倬[1] 

机构地区：[1]青岛大学医学院附属青岛市立医院心脏外科,山东青岛266011

出　　处：《中国体外循环杂志》2005年第2期84-86,共3页Chinese Journal of Extracorporeal Circulation

基　　金：青岛市科委资助项目(2001KNS-2E-51)

摘　　要：目的研究供心常温不同时间热缺血后细胞凋亡的变化。方法利用猪原位心脏移植实施方法,18只猪随机分为3组,对照组(C,n=6)灌注低温保护液后切取供心,4℃保存4h;热缺血1组(E1,n=6)常温阻断主动脉缺血5min后灌注低温保护液后切取供心;热缺血2组(E2,n=6)常温阻断主动脉缺血10min后,灌注低温保护液后切取供心。三组低温保存供心4h后分别行原位心脏移植。供心移植2h后取左心室心肌检测心肌细胞凋亡的变化。结果供心移植2h后,E1、E2组心肌细胞凋亡明显高于C组(P<0.01),E2组心肌细胞凋亡明显高于El组(P<0.01)。结论供心热缺血时间的延长增加移植后的心肌细胞凋亡的发生。OBJECTIVE To investigate the expression of apoptosis on the different warm ischemic heart donor.METHODS The experimental models of pig orthotopic heart transplantation were established 18 pigs were randomly divided into control group (C),Warm ischemia 1 group (E1) and warm ischemia 2 group (E2).Group C, the donor hearts were removed after perfused hypothermia protection and preserved 4h at 4 ℃. Group E1,the donor hearts were removed 5 min aftet aortic clamping and presered 4h at 4℃. Group E2,the donor hearts were removed 10 min after aortic clamping and preserved 4h at 4℃. Furthermore, the donor hearts werer transplated. The terminal transferase UTP nick end labeling (TUNEL) was detected. RESULTS The myocardial apoptosis index significantly increase in E1 and E2 group than C group. CONCLUSION This study demonstracted that the donor heart warm ischemia increase myocardial cell apoptosis.

关 键 词：心脏移植 热缺血 细胞凋亡 

分 类 号：R654.2[医药卫生—外科学]