电针抗吗啡戒断大鼠胸腺细胞凋亡的实验观察  

Experimental Study on the Effect of Electroacupuncture in Resisting Apoptosis of Thymocytes in Rats with Morphine Abstinence Syndrome

作　　者：张荣军[1] 宋小鸽[1] 唐照亮[1] 侯晓蓉[1] 陈全珠[1] 许冠荪[1] 陈亦宜[1] 

机构地区：[1]安徽中医学院针灸经络研究所,合肥230038

出　　处：《针刺研究》2005年第2期94-96,共3页Acupuncture Research

基　　金：安徽省重点科研项目(00022048);合肥市重点科研项目(01038)

摘　　要：目的:观察电针对吗啡戒断大鼠免疫器官及胸腺细胞凋亡的影响。方法:连续15d肌肉注射递增量吗啡,建立大鼠慢性吗啡成瘾模型,观察电针对吗啡戒断大鼠免疫器官胸腺、脾脏及其指数的影响,运用TUNEL法标记各组大鼠胸腺的凋亡细胞。结果:与正常组比,戒断组大鼠免疫器官胸腺、脾脏萎缩,重量下降(P<0.01),胸腺细胞凋亡率升高;电针组大鼠胸腺、脾脏萎缩减轻,重量上升(P<0.01),胸腺细胞凋亡率下降。结论:电针“足三里”穴具有保护免疫器官、抑制胸腺细胞凋亡的作用。Objective: To investigate the effect of electroacupuncture (EA) on thymus and spleen and apoptosis of thymocytes in morphine withdrawal rats. Methods: Rat abstinence syndrome model was established by continuous muscular injection of morphine (with the injected doses increasing gradually from 4 mg to 140 mg/kg, 1～3 times/d) to the hind limbs for 15 days. A total of 24 Wistar rats were randomly and evenly assigned to normal control group, abstinence syndrome (AS) group and EA (ST-36) group. The net weight of the thymus and spleen were detected and recorded.TUNEL method was applied to mark the apoptosis of thymocytes. EA (2～100 Hz, 1～2 mA) was applied to bilateral “Zusanli”(ST 36) for 30 min, once daily, continuously for 7days. Results: In comparison with normal control group, a significant decrease of the weight of both thymus and spleen, and the thymus index and spleen index was observed in AS group(P<0.01). Comparison between AS group and EA group showed that the weight of both thymus and spleen of EA group increased significantly than that of AS group, and both thymus index and spleen index of EA group were bigger than those of AS group (P<0.01), indicating that the effect of AS was reversed by EA. TUNEL method showed that the apoptosis rates of thymocytes in normal control, AS and EA groups were 5.7%, 51.1% and 31.8% respectively, displaying that EA could suppress AS-induced apoptosis of thymocytes. Conclusion: EA of ST-36 may protect the immune organ and inhibit the apoptosis of thymocytes, which may account, at least partly, for the suppression of EA on immune organ impaired by morphine.

关 键 词：电针 吗啡戒断大鼠 细胞凋亡 TUNEL法 

分 类 号：R245.97[医药卫生—针灸推拿学]