两种冷冻保护剂对形态正常精子百分率影响  

The effect of cryopreservative mediums on normal morphology rate of human spermatozoa.

作　　者：孙妍[1] 高元齐[1] 刘睿智[1] 薛百功[1] 宋玲[1] 

机构地区：[1]吉林大学基础医学院细胞生物学教研室,吉林长春130021

出　　处：《中国优生与遗传杂志》2005年第6期100-101,共2页Chinese Journal of Birth Health & Heredity

基　　金：吉林省科技厅资助项目(20030433)

摘　　要：目的比较甘油和甘油—卵黄—柠檬酸钠(GYC)两种冷冻保护剂对精子形态影响。方法应用甘油和GYC两种冷冻保护剂(CPM)对精液标本进行速冻和缓慢冷冻保存,应用计算机精液分析仪进行精子形态分析。结果冷冻复温后形态正常精子百分率与冷冻前比较的形态正常精子百分率比较明显下降(P<0.001);速冻与缓慢冷冻法中两种保护剂间形态正常精子百分率比较均无显著性差异(P>0.05)。结论冷冻保存易造成形态正常精子百分率下降,两种保护剂对精子形态没有影响。Objective: To study the effect of different cryoprotective mediums(CPMs) on normal morphology rate of human spermatozoa. Methods: Human semen sample were cryopreserved by fast frozen and slow frozen method. The sperm morphology were analyzed by automated sperm morphology analyzer(ASMA). Results: The normal morphology sperm rate was significantly decreased after freezing (P<0.001). There were no statistics differences (P>0.05) in normal morphology sperm rate between GYC and glycerol groups.Conclusion: Freezing could affect normal morphology sperm rated,GYC and glycerol could not affect normal morphology sperm rated.

关 键 词：人精子 冷冻保存 精子形态 冷冻保护剂 

分 类 号：R698.2[医药卫生—泌尿科学]