CXCL12在母胎界面的表达及其促进BeWo滋养细胞系的体外迁移作用  

CXCL12 increases the roles of BeWo human trophoblasts cell line migration in vitro.

作　　者：王轶英[1] 邹丽[1] 

机构地区：[1]华中科技大学附属协和医院妇产科,武汉430022

出　　处：《中国优生与遗传杂志》2005年第6期22-23,F004,共3页Chinese Journal of Birth Health & Heredity

摘　　要：目的研究CXCL12在早孕期母胎界面的表达情况及其对BeWo细胞的体外迁移作用。方法免疫组织化学法检测早孕期绒毛及蜕膜组织中CXCL12的表达情况;用Transwell细胞侵入系统检测CXCL12对BeWo细胞迁移的影响。结果早孕期绒毛及蜕膜组织均有CXCL12的表达,分布于绒毛柱、滋养细胞及间质血管内;CXCL12对BeWo细胞具有趋化作用,浓度达10ng/ml时趋化效应最强。结论CXCL12可促进BeWo细胞的迁移,因此早孕期母胎界面产生的CXCL12可能参与滋养细胞的迁移,对于维系正常妊娠发挥了一定作用。Objective: To investigate the expression of CXCL12 at maternal-fetal interface in early pregnancy and the roles of CXCL12 on BeWo cell line migration. Methods: To detect the expression of CXCL12 in villi and decidual tissue by immunohistochemistry; to examine the effect of CXCL12 on BeWo cell line migration by Transwell system. Results: CXCL12 were detected in the villi and decidual tissue and distributed in villous column, trophoblasts and blood vessels;CXCL12 played roles on the migration of BeWo cell line and it appeared the maximum chemotactic effect at the density of 10ng/ml. Conclusion: CXCL12 could increase the migration of BeWo cell line. Therefore, CXCL12 produced by maternal-fetal interface in human first trimester pregnancy may contribute to the migration of trophoblasts and play roles on nomal pregnancy.

关 键 词：母胎界面 迁移作用 体外 细胞系 表达及 免疫组织化学法 表达情况 蜕膜组织 滋养细胞 早孕期 细胞迁移 系统检测 趋化作用 正常妊娠 绒毛 L细胞 血管内 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]