莫桑比克非鲫卵壳膜形成的电镜观察  

ELECTRON MICROSCOPIC OBSERVATIONS ON THE FORMATION OF CHORION (EGG ENVELOPE)OF ORECHROMIS MOSSAMBICUS

作　　者：王爱民[1] 

机构地区：[1]新疆大学生物系,乌鲁木齐830046

出　　处：《海洋与湖沼》1994年第4期385-389,T001,共6页Oceanologia Et Limnologia Sinica

摘　　要：于1986年9－12月对取自湖北省水产研究所实验鱼网箱中的莫桑比克非鲫，运用透射电镜观察卵子发生中印壳膜的形成过程。结果表明，随着卵母细胞的生长，在卵母细胞和颗粒细胞之间先后出现3层结构，即Z1，Z2和Z3；初步确定：Z1可能由颗粒细胞分泌形成，z2和z3由印母细胞分泌形成，最终z1，z2和Z3融为一体成为卵壳膜。对印亮膜的结构特征及其与特殊的生殖习性——衔卵孵化的关系的分析认为，衔卵孵化导致卵壳膜的厚度降低。The formation of chorion (egg envelope) of Orechromis mossambicus which was offered by Hubei Fisheries Research Institute has been observed by transmission electron microscope from September to December 1986. The results are as follows:1.The structure of chorion undergoes several changes when it is formed during oocyte development. A trilaminar appearance is produced successively. For conveience of description, the portions are referred to Zone 1,2 and 3 respectively. Zone 1 first appears as a homogeneous electron-lucent layer between the microvilli of oocyte and macrovili of granulosa cells. Zone 2 is immediately beneath Zone l, and consists of an extremely electron apaque materials. Zone 3 is subjacent to Zolle 2. It is the largest and most complex of the three zones, and consists of amorphous ma-terials organized in a reticular-like network. It has been preliminaryly confirmed that Zone 1 is formed by granulosa cells, Zone 2 and 3 are secreted by oocyte itself with the dense core vesicles.2.During the late period of oocyte growth, the chorion compacts. As the com-paction occurs, Zone 1 turn into a lot of small pieces, Zone 2 becomes a thin dense layer, and Zone 3 becomes a thick multiple lamellae. The main part of the chorion is composed of the Zone 3.3.Thickness of the chorion in O. mossambicut is 4.5μm and it is thinner than those of other fresh water teleosts which have been studied. The reduction of thick ness in the chorion is closely related to the special reproductive manner, that is,oral hatching embryos.

关 键 词：莫桑比克非鲫 卵壳膜 透射电镜 

分 类 号：Q959.468[生物学—动物学]