青海湖裸鲤繁殖群体遗传多样性的RAPD分析  

Genetic diversity analysis of different populations of Gymnocypris przewalskii by RAPD

作　　者：张春霖[1,2] 陈大庆[3,2,4] 史建全[5] 祁洪芳[5] 鲁成[6] 

机构地区：[1]西南农业大学水产学院 [2]中国水产科学研究院长江水产研究所农业部淡水鱼类种质资源与生物技术重点开放实验室,湖北荆州434000 [3]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室 [4]中国水产科学研究院淡水渔业研究中心,江苏无锡214081 [5]青海省鱼类原种良种场 [6]西南农业大学农业部蚕桑学重点实验室

出　　处：《水产学报》2005年第3期307-312,共6页Journal of Fisheries of China

基　　金：青海省科技厅"十五"攻关项目(2001-N-122);淡水生态与生物技术国家重点实验室项目(2003FB02)

摘　　要：采用随机扩增多态性DNA(RAPD)方法对青海湖裸鲤的3个洄游繁殖群体-黑马河(HM)、布哈河(BH)及沙柳河(SL)群体各30个个体的DNA多态性进行了分析。用13个引物在三个群体中共检测出85个位点,其中多态性位点68个。青海湖裸鲤群体总的DNA多态位点百分率为80.0%。数据分析结果显示:青海湖裸鲤3个群体总的Nei基因多样性为0.3395,Shannon遗传多样性信息指数为0.4861,存在着较为丰富的遗传多样性。青海湖裸鲤3个群体间平均遗传距离为0.0788,基因分化系数Gst为0.1070,表明3个繁殖群体间产生了一定程度的遗传分化,通过UPGMA方法进行聚类分析显示,HM群体和BH群体优先聚类,表示它们之间的基因交流程度要高于它们分别与SL群体之间的交流。DNA polymorphism of thirty individuals of each of the three migrating reproductive populations of Gymnocypris przewalskii (Kessler) in the Qinghai Lake was analyzed by random amplified polymorphic DNA (RAPD), which were Heimahe population (HM), Buhahe population (BH) and Shaliuhe population (SL). A total of 85 loci, including 68 polymorphic ones, were detected from the three populations by using 13 primers. The average percentage of polymorphic loci to total DNA of the three populations was 80.0%. The results of data analysis showed that the populations had on average the Nei's value of 0.3395 for gene diversity and the Shannon's information index of 0.4861, there existing a relatively abundant genetic diversity. The average genetic distance among the three populations was 0.0788. The gene differentiation coefficient (Gst) of 0.1070 indicated that there was genetic differentiation of a certain degree among the three populations. Clustering analysis by UPGMA method showed that HM and BH were clustered with priority, indicating that the gene flow of both populations was more frequent than that between either and SL.

关 键 词：青海湖裸鲤 DNA多态性 遗传多样性 遗传分化 基因交流 

分 类 号：Q347[生物学—遗传学] S917[农业科学—水产科学]