补阳还五汤中生物碱类化合物的分离与结构鉴定  

Alkaloids isolated from Buyang Huanwu Decoction and their structure identification

作　　者：吴军[1] 屠鹏飞[1] 赵玉英[1] 

机构地区：[1]北京大学中医药现代研究中心药学院,北京100083

出　　处：《中草药》2005年第7期965-968,共4页Chinese Traditional and Herbal Drugs

基　　金：国家"九五"攀登项目(970211018)

摘　　要：目的研究补阳还五汤的抗血栓有效部位及其活性成分.方法用多种色谱、核磁共振波谱和单晶X-衍射方法对补阳还五汤水煎液的生物碱类化合物进行了系统的分离和结构鉴定.结果分离并鉴定了4个生物碱类化合物,分别为N-(3′-马来酰亚胺)-5-羟甲基-2-吡咯甲醛(Ⅰ)、4-氨甲酰基-2-吡咯甲酸(Ⅱ)、N-(1′-D-去氧木糖醇基)-6,7-二甲基-1,4-二氢-2,3-喹喔啉二酮(Ⅲ)及2,3,4,9-四氢-1H-吡啶骈[3,4-b]吲哚-3-羧酸(Ⅳ).结论以上化合物均首次从补阳还五汤中分离得到且化合物Ⅰ～Ⅲ为新化合物.Objective To study the antithrombotic compounds and bioactive fraction from Buyang(Huanwu) Decoction(BHD).Methods Four alkaloids have been isolated from BHD by repeated chromatographies.Their structures were elucidated by means of spectral and single-crystal Xray diffraction analysis.Results They are N-(3′-maleimidyl)-5-hydroxymethyl-2-pyrrole formaldehyde(Ⅰ),4-carbamoyl-2-pyrrole carboxylic acid(Ⅱ),N-(1′-D-deoxyxylitolyl)-6,7-dimethyl-1,4-dihydro-2,3-quinoxalinedione(Ⅲ),and 2,3,4,9-tetrahydro-1H-pyrido indole-3-carboxylic acid(Ⅳ).Conclusion All these compounds are isolated from BHD for the first time and compounds Ⅰ-Ⅲ are new alkaloids.

关 键 词：补阳还五汤 生物碱 N-(3’-马来酰亚胺)-5-羟甲基-2-吡咯甲醛 4-氨甲酰基-2-吡咯甲酸 N-(1'-D-去氧木糖醇基)-6 7-二甲基-1 4-二氢-2 3-喹喔啉二酮 

分 类 号：R284.1[医药卫生—中药学]