大鼠习瓣膜含神经肽Y血管性肠肽P物质和降钙素基因相关肽神经的分布及年龄变化  

Distribution of Peptide -Containing Nerves in Cardiac Valves of Rats with Different Ages

作　　者：毛秦雯[1] 苏慧慈[1] 张远强[1] 黄威权[1] 

机构地区：[1]第四军医大学组织学胚胎学教研室

出　　处：《解剖学报》1994年第1期65-69,T013,共5页Acta Anatomica Sinica

摘　　要：用免疫组织化学ＡＢＣ法结合葡萄糖氧化酶－ＤＡＢ－硫酸镍铵增强技术，研究了１月、８月和１８月龄大鼠心瓣膜中ＮＰＹ、ＶＩＰ、ＣＧＲＰ和ＳＰ免疫反应阳性神经纤维的分布。结果显示，以上４种含肽神经纤维在８月龄大鼠房室瓣上均有分布，密度顺序为ＮＰＹ＞ＣＧＲＰ＞ＶＩＰ和ＳＰ，含ＶＩＰ和ＳＰ神经纤维的密度相近。三尖瓣中含ＮＰＹ和ＳＰ神经纤维的密度大于二尖瓣，含ＶＩＰ和ＣＧＲＰ神经纤维的密度与二尖瓣的相近。瓣膜中的含肽神经纤维可从瓣膜附着处的心壁及乳头肌内的神经两个方向延伸而来。主动脉和肺动脉瓣内仅发现有ＮＰＹ神经。随大鼠年龄增长，房室瓣中含ＮＰＹ神经的密度有增加趋势。含ＣＧＲＰ、ＶＩＰ、ＳＰ神经在１月龄大鼠房室瓣上的密度为零，在８月和１８月储时的密度与１月龄时的差异非常显著，８月和１８月龄时的密度间无明显差异。１月龄和１８月龄大鼠的主动脉和肺动脉瓣上，未见到４种含肽神经。我们的结果为心瓣膜的神经支配提供了新的资料，但这些含肽神经在心瓣膜的作用尚待探讨。n this work free-floating immunostaining with ABC method combined with nickel enhance-ment tecknique was used to demonstrate NPY-, VIP- , CGRP-and SP-immunoreactive nervefibers in the cardiac valves of rats aged 1 , 8 and 18 months. The atrioventricular valves wererichly innervated by immunopositive fibers. The sequence of the density of the four peptide-con-taining nerves was NPY>CGRF>VIP and SP. There was no significant difference between SP and VIP tontaining fibers. More NPY- and SP- containing fibers were found in the right atri-oventricular valves than in the left ones. No obvious difference was observed in the densities ofVIP- and CGRP- containing fibers between the right and left atrioventricular valves However,in the semilunar valves only NPY-immunoreactive fibers could be seen. Two origins of the fiberswere noticed to enter into the atrioventricular valves: (1) from the cardiac wall to which thevalves were attached , and (2), from the chordae tendineae in whith the nerve fibers were extend-ed from the papillary muscles and traveled along their longitudinal axes. In the atrioventricularvalves the densities of the ppptide-containing nerves increased with aging. Our lindings addednew data to the innervation of the cordiac valves, but the role of these nerves remains to beelacidated.

关 键 词：神经分布 肽能神经 免疫组织化学 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学]