胎鼠和幼鼠运动神经元诱向因子的免疫组织化学定位  

IMMUNOHISTOCHEMICAL LOCALIZATION OF MOTONEURONOTROPHIC FACTOR IN FETAL AND NEONATALRATS

作　　者：周明华[1] 黄中新[1] 吴玺印[1] 卢乃湄[1] 饶晓黎[1] 

机构地区：[1]香港大学医学院解剖学系,香港暨南大学医学院组织学与胚胎学教研室

出　　处：《解剖学报》1994年第2期189-192,T015,共5页Acta Anatomica Sinica

基　　金：本研究由香港大学和中华医学基金

摘　　要：用免疫组织化学ＡＢＣ法检测了ＳＤ大鼠胚胎（Ｅ１３、１４、１８、２０ｄ）和幼鼠（Ｐ１、３、６ｄ；２周、３周）体内运动神经元诱向因子（ＭＮＴＦ）的分布。结果表明，大鼠Ｅ１８ｄ之前的胚胎组织或脏器的ＭＮＴＦ－ＭｃＡｂ免疫组织化学反应均为阴性。在大鼠Ｅ１８ｄ的心肌、骨骼肌呈免疫阳性反应，而平滑肌为阴性反应；肝、肾、脊髓的边缘层和套层的原始神经细胞、胶质细胞及Ｅ２０ｄ前角运动细胞均为阳性反应。从Ｐ１ｄ到３周龄大鼠的心肌、骨骼肌仍为阳性反应；肝和肾的阳性反应逐渐减弱；胰腺泡上皮细胞为阴性反应，但胰岛内分泌细胞免疫阳性反应明显，而且随着动物成长其阳性反应逐渐增强，直至成体大鼠的胰岛才转为阴性反应。在３周龄动物，主要是脊髓前角运动神经元有明显的阳性反应。实验结果表明，ＭＮＴＦ在ＳＤ大鼠胚胎晚期才出现，而且分布范围比较局限。提示ＭＮＴＦ在体内的主要功能为促进其效应细胞（运动神经元）的生长、成熟和行使功能。In this paper monoclonal antibody specific to motoneuronotrophic factor was used to study the immunohistochemical distribution of the motoneuronotrophic factor in embryos(E13,14,18and 21 days)and neonates(P1,3,6,14 and 21 days)of SD rats.The results revealed that viscera and tissues from embryos younger than 18 days were immunoreactively negative;those from embryos 18 days onward including cardiac and skeletal muscles, liver and kidney,and primitive neurocytes and neuroglia present in the marginal and mantal layers of the spinal cord,as well as the anterior horn motoneurons in the E20 spinal cord were immunoreactively positive;but not the smooth muscles.For neonatal rats from 1 to 21 days old,their cardiac and skeletal muscles were still immunoreactively positive and their liver and kidney were gradually becoming weakly positive.Their pancreatic acinar cells were negatively stained,however,the pancreatic islet cells were distinctively becoming strong immunoreactively positive until in adult rat,and then became immunoreactively negative.The spinal cord was weakly immunoreactive positive in newborn rats but the immunoreactivity in its anterior horn motoneurons became distinctively strong positive in 21 day old rats.These results indicated that the motoneuronotrophic factor began to be expressed quite locally during the late embryonic period and later more concentrated in the neuromuscular system,and suggested that the major role of the motoneuronotrophic factor is mainly involved in promoting the growth,maturation and function of the motoneurons.

关 键 词：运动神经元 诱向因子 免疫组织化学 

分 类 号：R321.5[医药卫生—人体解剖和组织胚胎学]