鉴别伪狂犬病病毒强、弱毒株单克隆抗体的制备  

Preparation of the monoclonal antibodies discriminating virulent and attenuated strains of psudorabbies virus (PRV)

作　　者：程晓霞[1] 陈少莺[1] 胡奇林[1] 俞伏松[1] 朱小丽[1] 陈仕龙[1] 林锋强[1] 欧阳岁东[1] 车勇良[1] 林天龙[1] 程由铨[1] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福建福州350013

出　　处：《福建农业学报》2005年第2期87-90,共4页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划项目(2002N048)

摘　　要：用纯化的猪伪狂犬病病毒闽A株(PRV-FA)免疫Balb/c鼠,取脾细胞和骨髓瘤细胞进行细胞融合,经间接ELISA筛选,获得11株能稳定分泌伪狂犬病毒单克隆抗体的杂交瘤细胞株,其中6株仅与强毒FA反应,而不与弱毒FB、Bartha及gE缺失疫苗株发生反应。特异性鉴定结果表明,各株单抗均不与猪瘟病毒、猪流感病毒、猪呼吸繁殖障碍综合症病毒、猪细小病毒等发生交叉反应。The purified psudorabies virus strain FA (PRV -FA) was inoculated to Balb/c mice. Eleven monoclonal hybridoma cell lines which were able to stably excrete monoclonal antibodies against PRV were produced by means of hybridoma technique and indirect ELISA detection. The results of immunologic characteristics identification revealed that 6 hybridoma cell lines only reacted with PRV strain FA and didn't react with PRV-FB, PRV-gE-and PRV -Bartha. And all these monoclonal antibodies didn't react with HCV, SI, PRRSV and PPV.

关 键 词：伪狂犬病病毒 强弱毒株 单克隆抗体 制备 

分 类 号：S852.65[农业科学—基础兽医学] S858.28[农业科学—兽医学]