水稻幼苗中胚轴长度QTL及与Fe^(2+)浓度的互作效应的遗传分析  

QTL Mapping and Interaction Analysis of Genotype ×Environment (Fe^(2+)-concentrations ) for Mesocotyl Length in Rice (Oryza sativa L.)

作　　者：欧阳由男[1] 张秋英[1] 张克勤[1] 禹盛苗[1] 庄杰云[1] 金千瑜[1] 程式华[1] 

机构地区：[1]中国水稻研究所水稻生物学国家重点实验室/国家水稻改良中心,杭州310006

出　　处：《Acta Genetica Sinica》2005年第7期712-718,共7页

基　　金：国家重大基础研究前期研究专项基金(编号:2002CCA04100);浙江省重点项目基金(编号:021102169);浙江省自然科学基金(编号:M303373)~~

摘　　要：应用珍汕97B/密阳46的RIL群体及其构建的分子连锁图谱,在4个浓度的FeSO4处理下发芽,测定中胚轴长度。采用QTLMapper基因定位软件检测控制中胚轴长度的加性效应QTLs和加性×加性上位性QTLs,分别在1、2、3、4、5、6、7、8、9、11、12等染色体上定位了27个控制中胚轴长度的QTLs,其中在第1、5、9等3条染色体上定位了6个具有加性效应的QTLs。在CK和FeSO41.79mmol/L浓度下,在第5染色体长臂相邻区间检测到1个加性效应QTL,其增效等位基因来自于父本密阳46,它能使中胚轴伸长0.042cm(CK)、0.172cm(1.79mmol/L),贡献率分别为4.3%和11.4%;在高浓度FeSO4(7.16mmol/L和14.32mmol/L)下分别在1、5、9染色体上检测到的4个加性效应QTL,贡献率为3.5%～10.4%,3个QTL中来自于母本珍汕97B的等位基因使中胚轴伸长0.024～0.046cm,1个QTL来自父本的增效等位基因使中胚轴伸长0.033cm。同时在第5染色体长臂上检测到具有显著的加性效应与Fe2+互作效应的QTL1个。A recombinant inbred lines (RILs) population derived from a cross between Zhenshan97B and Mi- yang46 was used for detecting QTLs with additive effects and additive-by-additive epistasis for rice mesocotyl length.A linkage map consisting of 207 DNA markers,distributing on the 12 chromosomes of rice,was employed for QTL mapping by using software QTL Mapper 1.6 of mixed linear model.Rice mesocotyl length under germination conditions with 4 different FeSO4 concentrations (0,1.79,7.16,14.32 mmol/L) was measured 7 days after planting.A total of 6 QTLs with significant additive effects on chromosome 1,5 and 9,with variance explained of 3.5%-11.4%,eleven QTLs with significant additive×additive epistatic effects on chromosome 1,2,3,4,5,8 were detected,with variance explained of 4.5%-8.1%.In addition,one QTL for environmental interaction (Fe^2+-concentrations) was detected.

关 键 词：水稻 中胚轴长度 数量性状座位 基因型×硫酸亚铁浓度互作 

分 类 号：S511[农业科学—作物学]