火炬松原生种源和引种群体RAPD遗传多样性  

RAPD analysis on the genetic diversity of original provenances and internal populations of Pinus taeda

作　　者：税珺[1] 黄少伟[2] 陈炳铨[2] 

机构地区：[1]江门市林业科学研究所,广东江门529000 [2]华南农业大学林学院,广东广州510642

出　　处：《华南农业大学学报》2005年第3期74-76,81,共4页Journal of South China Agricultural University

基　　金："九五"国家科技攻关项目(9501103183);广东省科技攻关项目(962203309)

摘　　要：利用12个10bp随机引物对火炬松Pinustaeda19个原生种源和国内早期引进的5个群体进行RAPD扩增.结果表明,火炬松原生种源和国内引种群体的遗传多样性均十分丰富,RAPD多态性位点的比率分别为81.3%和99.2%.原生种源中共检出18条特异性谱带,分属于13个种源,另有5个种源存在特异性缺失谱带,共10条,这些特异性谱带和特异性缺失谱带可作为种源鉴别的重要依据.根据原生种源特异性谱带在不同国内群体中出现的频率,推测国内分3次引入的群体起源比较一致,主要集中于来自美国的北卡罗来纳、佛罗里达和密西西比的4个种源.Twelve 10 bp random primers were used to amplify DNA samples from 19 original provenances and 5 previously introduced populations of loblolly pine (Pinus taeda L.). It was found that the levels of genetic diversity were very high for both original provenances and previously introduced populations by means of RAPD polymorphic locus rate, which were 81.3% and 99.2% respectively. Eighteen specific bands were detected from 13 of the original provenances. Ten specific bands were lost in 5 of the original provenances. These specific bands and particularly lost bands can be the important basis in the determination of provenance. Based on the distribution frequency of specific bands from original provenances, it was deduced that, although the internal populations were introduced in 3 times, the sources were relatively consistent. They mainly originated from 4 provenances in North Carolina, Florida and Mississippi of USA.

关 键 词：RAPD 火炬松 种源 群体 遗传多样性 

分 类 号：S722.31[农业科学—林木遗传育种]