高效液相色谱法测定人血浆中异烟肼及乙酰异烟肼浓度  

Determination of Isoniazid and Acetylisoniazid Concentration in Human Plasma by HPLC

作　　者：许亦鸣[1] 陈冰[2] 王洁[1] 周秋云[1] 朱文玉[1] 李金恒[2] 

机构地区：[1]南京市胸科医院药剂科,南京市210029 [2]南京军区南京总医院临床药理科,南京市210002

出　　处：《中国药房》2005年第13期1001-1003,共3页China Pharmacy

摘　　要：目的:建立以高效液相色谱法测定人血浆中异烟肼(INH)及其代谢物乙酰异烟肼(AcINH)浓度的方法。方法:血浆加入三氯醋酸沉淀蛋白后,将上清液分为2份,一份加入蒸馏水,另一份加入盐酸,80℃孵育1h后,分别加入1%桂皮醛进行柱前衍生化。色谱柱为HypersilBDSC18,流动相为0.02mol/L磷酸二氢钾(pH=4.0)-乙腈(69∶31),检测波长为340nm,流速为1ml/min。结果:INH检测浓度在0.57～145.88μmol/L范围内线性关系良好,INH和AcINH平均回收率分别为101.1%、101.5%,日内、日间相对标准差均<10%。结论:本法可用于异烟肼血药浓度监测及乙酰化酶代谢能力研究。OBJECTIVE:To establish a HPLC method for determination of the plasma concentrations of isoniazid(INH)and its metabolite acetyl-isoniazid(AcINH).METHODS:Trichloroacetic acid was added to plasma to precipitate protein,then the supernatant was divided into 2 parts,distilled water was added to the first part;while HCl was added to the second one,after incubated at 80℃for 1 hour,1%cinnamaldehyde was added to perform precolumn derivatization.The column was Hypersil BDS C 18 ,the mobil phase was eluted with0.02mol/L NaH 2 PO 4 (pH=4.0)-acetontrile(69∶31)and detected wave-length was 340nm,the flow fate was1ml/min.RESULTS:INH detectable concentration showed a good linear correlation in the range of 0.57～145.88μmol/L,the average recoveries of INH and AcINH were 101.1% and 101.5% respectively,the average RSD within or between day was less than 10%.CONCLUSION:The present method is applicable to monitoring of INH plasma concentration and the study of metabolism capacity of N-acetyltransferase.

关 键 词：异烟肼 乙酰异烟肼 高效液相色谱 柱前衍生化 

分 类 号：R978.3[医药卫生—药品] R927.2[医药卫生—药学]