无籽西瓜子叶离体培养及植株再生系统的建立  

Tissue Culture and Plantlet Regeneration from Cotyledon of Citrullus Lanatus

作　　者：栗燕[1] 郑先波[1,2] 张恒涛[2] 夏国海[1] 宋尚伟[1] 

机构地区：[1]河南农业大学林学园艺学院,河南郑州450002 [2]中国农业大学农学与生物技术学院

出　　处：《莱阳农学院学报》2005年第2期118-121,共4页Journal of Laiyang Agricultural College

基　　金：河南省杰出人才创新基金项目(200203056)

摘　　要：以无籽西瓜黑蜜5号无菌苗子叶为外植体,对其离体培养及植株再生的条件进行了详细的研究。研究结果表明:子叶芽诱导的最佳培养基配方为MS+6.BA3.0mg/L+NAA0.1mg/L。芽的诱导需要先在黑暗条件下启动,生长分化则须在光下进行。适宜的生根培养基配方为1/2MS+IAA0.4mg/L和1/2MS+IBA0.3mg/L。试验初步建立了适用于无籽西瓜遗传转化和快速繁育的子叶再生系统。The plantlet regeneration from Cotyledon of Citrullus lanatus was studied. The results showed that the most suitable medium for buds induction from cotyledon of Citrullus lanatus was MS + 6-BA 3. Omg/L + NAA 0. Img/L. The buds induction of the cotyledon should be started in darkness and its further growth and differentiation must be carried out in light. The optimum rooting media were 1/2MS + IAA 0. 4mg/Land 1/2MS + IBA 0. 3mg/L. The research preliminarily established a system of genetic transformation and quick propagation for cotyledon of Citrullus Lantus.

关 键 词：无籽西瓜 子叶 组织培养 植株再生 

分 类 号：Q943.1[生物学—植物学]